��
��
��
Abstract
��
Vol 48 No. 10: 923-929 [PDF] [Full Text]
��
MiR-98 promotes chondrocyte apoptosis by decreasing Bcl-2 expression in a rat model of osteoarthritis
��
Jing Wang1,†, Lingqiang Chen2,†, Song Jin1, Jun Lin1, Hongmei Zheng1, Hong Zhang1, Hongtao Fan1, Fang He1, Sha Ma1 and Qin Li1,*

1Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China
2Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
These authors contributed equally to this work.
��

Abstract  Altered expression of miRNA-98 (miR-98) has been reported in osteoarthritis (OA) patients, while its role and underlying mechanisms remain elusive. In the present study, a rat model of OA was established using modified Hulth method, and the expression level of miR-98 and its effect on cartilage degradation and cell apoptosis in OA rats were examined. The results showed that up-regulated miR-98 was observed in OA rats, and knockdown of miR-98 in OA rats resulted in an inhibitory effect on cartilage degradation and chondrocyte apoptosis. Then the potential apoptosis associated genes regulated by miR-98 were screened and examined in cartilage tissues. The target gene of miR-98 was validated by luciferase reporter assay. The data showed that the increased miR-98 was accompanied with a reduced expression of Bcl-2 at both mRNA and protein levels. Furthermore, the silencing of miR-98 in OA rats prevented the down-regulation of Bcl-2 in cartilage tissues. Finally, the luciferase reporter assay validated that Bcl-2 was the target gene of miR-98. In this study, we found that miR-98 might promote chondrocyte apoptosis and cartilage degradation by down-regulating Bcl-2 expression in the pathogenesis of OA, suggesting that miR-98 can be a potential target for the treatment of OA.

��

Keywords   microRNA-98; osteoarthritis; Bcl-2; apoptosis

��

Received   2016-3-11����
Accepted  
2016-7-26

��

Funding  This work was supported by the grant from the National Natural Science Foundation of China (No. 81260286).

��

* Correspondence address  Tel/Fax: +86-871-63638784; E-mail: [email protected]

��
Browse:210
��
Manuscript Submission

    ScholarOne Manuscripts

User ID

Password

           

    Password Help

E-Mail

��
��
Copyright 1999-2016��Acta Biochimica et Biophysica Sinica��All Rights Reserved
Address: Room 407, Building 31B, 319 Yueyang Road, Shanghai, 200031 P.R. China
Fax: 86-21-54920954��Email: [email protected]
��
��ICP��05033115����| �����ǵ� λ�����ߣ���ӭ��