Vol 49 No. 4: 311-317 [PDF] [Full Text]
Enhanced tenogenic differentiation and tendonlike tissue formation by CHIP overexpression in tendon-derived stem cells
Weifeng Han1, Lei Chen2, Junpeng Liu3, and Ai Guo1,*

1Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China,
2Institute of Orthopaedics, The First Affiliated Hospital of Chinese People’s Liberation Army General Hospital, Beijing 100048, China, and
3Department of Orthopaedics, Air Force General Hospital, People’s Liberation Army of China, Beijing 100142, China

Abstract  The carboxyl terminus of Hsc70-interacting protein (CHIP, also known as STUB1) plays critical roles in the proliferation and differentiation of many types of cells. The potential function of CHIP in tendon-derived stem cells (TDSCs) remains largely unknown at present. Here, we investigated the effects of CHIP on tenogenic differentiation of TDSCs via lentivirus-mediated overexpression. Forced expression of CHIP induced morphological changes and significantly enhanced cell proliferation, as well as tendon differentiation in vitro. Upon stimulation with differentiation induction medium, CHIP-overexpressing TDSCs displayed significant inhibition of differentiation into osteogenic and adipogenic lineages. Subsequent implantation of TDSCs overexpressing CHIP with collagen sponges into nude mice induced a marked increase in ectopic tendon formation in vivo, compared with the control group. Our findings collectively suggest that CHIP is an important contributory factor to tenogenic tissue formation.


Keywords   differentiation, tendon, stem cells, CHIP


Received   2016-10-25  


Funding  This work was supported by the National Natural Science Foundation of China (No. 81301557).


* Correspondence address  Tel: +86-10-63014411; Fax: +86-10-63023261; E-mail: docguoai8@sina.com

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