Progress in Targeted Gene Integration

LI Zhu-Hong, LIU De-Pei^* and LIANG Chih-Chuan
(National laboratory of Medical Molecular Biology, Institute of Basic Medical Sc iences,
Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100005, China )

Abstract　　The ultimate goal of gene therapy is correction of the genetic disor ders by introducing the genetic materials into targeted cells, and gene expressi on should be permanent and at an appropriate level. The widely used gene therapy vectors nowadays can not meet the demands. The targeted integration mediated by the homologous recombination is the best strategy for human gene therapy, becau se the gene expression is controlled by the natural elements. The progress in ho mologous recombination recently made it possible to correct the genetic disorder s by targeted integration of the interest gene.
Key Words　　Gene therapy； integration； homologous recombination

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