Cloning, Sequencing and Expression of Human Copper,
Zinc-superoxide Dismutase cDNA

SHI Hui-Juan, FAN Li-Qiang, WEI Dong-Zhi and YUAN Qin-Sheng^*
( Research Institute of Biochemistry, State Key Laboratory of Bioreactor Engineering,
East China University of Science and Technology, Shanghai 200237, China )
SUN Jian-Xing and WU Xiang-Fu
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China )

Abstract　　The cDNA encoding the human copper,zinc-superoxide dismutase(Cu,Zn-SOD) was amplified from the human liver by RT-PCR and sequenced. The cloned human Cu,Zn-SOD cDNA was ligated into expression vector pET-22b(+) under T7 promotor. After 3 h induction with 1 mmol/L IPTG, human Cu,Zn-SOD was highly expressed in E.coli BL21(DE3). The expression product was up to 30% of the total protein of the bacteria in soluble form, which had specific SOD activity.
Key Words　　Copper,zinc-superoxide dismutase; expression vector; gene cloning; gene expression; E. coli

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