Peptide Mapping and Primary Structure Analysis of hEGF
by Mass Spectrometry

ZENG Rong, SHAO Xiao-Xia and XIA Qi-Chang^*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China )

Abstract　　Liquid chromatography-mass spectrometry(LC-MS) was adopted to analyze peptide mapping of the hEGF digested by TPCK-trypsin and V8 to determine the molecular weight of each peptide fragment. Most of the peptide fragments were sequenced by tandem MS(MS-MS) to obtain the amino acid sequence. In addition, the number of disulfide bonds was confirmed. This method will facilitate the determination and structural analysis for nature, synthesis and recombinant proteins and peptides. The accuracy and resolution are both better than that of the routine method.
Key Words　　Liquid chromatography-mass spectrometry; MS peptide mapping; sequence analysis; hEGF

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