Modeling and Analysis of Structures of Phospholipase A₂'s
from Venom of Agkistrodon haly Pallas

LIANG Wei-Ping, ZHOU Yuan-Cong, WU Xiang-Fu and DING Da-Fu^*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China )

Abstract　　We have modeled three-dimensional structures of basic-acidic hybrid phospholipase A₂-II and neutral phospholipase A₂ from venom of snake Agkistrodon halys Pallas, based on the known structures of basic and acidic phospholipase A₂'s from the same source. We have compared these structures of phospholipase A₂'s, explained the results of fluorescent spectrum study on the phospholipase A₂'s and calculated the electrostatic potential maps on the catalytic active site. We suggest that the electrostatic potential around the catalytic active site of PLA₂ containing a calcium ion favors the binding of the PLA₂ to its substrate with negative charge.
Key Words　　Phospholipase A₂; homology modeling; electrostatic potential map

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