Expression of Brain-derived Neurotrophic Factor (BDNF) Gene
in Rat Myoblast Cells

LIU Jian, JIANG Qiong^*, GE Kai, SUN Lan-Ying, LIU Xin-Yuan and ZHENG Zhong-Cheng
( Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China )

Abstract　　Brain-derived neurotrophic factor (BDNF) gene was cloned into retrovirous vector plasmid pLNCX to form the pLNC/BDNF. After packaging in PA317 cell line, the infectious particles were used to infect rat myoblast cell line L-6TG. After selection with G418, the L-6TG/BDNF cells was collected. Results from Southern blot show that bdnf gene has successfully integrated into the L-6TG genomic DNA. The expression of bdnf gene has been proved by Northern blot and Dot blot. The content of BDNF in the culture medium of L-6TG/BDNF was about 25 ng·10^-6 cells per 24 h per ml.
Key Words　　Rat myoblast cell; bdnf gene; gene therapy

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