Efficient Secretion of Proteins Expressed
from Insect
Cells Directed by PAM Signal Peptide
JIANG Zhi-Hong, YANG Yu-Hong, WU Xiang-Fu, LI Bo-Liang* and
WANG De-Bao
( Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai
200031, China )
Abstract¡¡¡¡Signal and leading peptide
sequences of rat PAM was inserted into the baculovirus transfer vector, and
secretion expression plasmids pBACPAG2 and pBacPAI for the fusion gene
PABC-hGRF and PABC-IGF-I were constructed, respectively. By cotransfection with
linear genomic DNA of modified Autographa californica nuclear
polyhedrosis virus (BacPAK6) and homologous recombination, the recombinant
AcNPV, BacPAG and BacPAI, were obtained and identified. Fusion proteins
PABC-hGRF and PABC-IGF-I were secreted efficiently from Sf21 cells infected
with BacPAG and BacPAI, respectively, and those fusion protein could be
purified efficiently by IgG affinity column.
Key Words¡¡¡¡PAM signal peptide; insect
cell; excretion; efficient purification
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