Transformation of Neomycin Resistance Gene (neo^R)
into Silkworm (Bombyx mori.L.)

CHEN Xiu, ZHAO Yun¹, ZHANG Feng¹, PENG Wei-Ping,
FENG Xiao-Li¹, HUANG Jun-Ting and LU Chang-De^1*
( The Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China)
(¹Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China )

Abstarct 　　 Neomycin resistance gene (neo^R) flanked by 5′ and 3′ regions of fibroin H-chain gene of silkworm (Bombyx mori.L.) was transferred into eggs of silkworm by gene gun in the early period of fertilization. The larvae were fed with an artificial diet containing neomycin in early 24 hours post transfettion, and some of them survived. The neo^R encoding sequence in G₂ generation derived from the survivals was detected by Southern blotting. The results indicated that neo^R could be used as a selective marker for studies on transgenic silkworm.
Key Words　　Bombyx mori.L.; selective marker; neo^R; transgenic silkworm

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