Sequence Analysis of the Trailer Region of Wheat
Rosette Stunt Virus Genomic RNA

YE Yong-Jun and GONG Zu-Xun^*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China )

Abstarct 　　According to a highly conserved 8 amino acid sequence of L proteins in Rhabdoviridae, a 24-mer degenerate oligonucleotide primer was designed to amplify the 5′ terminus of the genomic RNA of wheat rosette stunt virus (WRSV), a plant rhabdovirus, by anchored PCR.The complete nucleotide sequence of the 5′ trailer in WRSV genomic RNA was determined by deoxynucleotide sequencing of cloned cDNA derived from the anchored PCR products.
Key Words　　Rhabdovirus; genome; trailer; LA-PCR

Corresponding author: Tel, 86-21-64746510; Fax, 86-21-64746510; e-mail, [email protected]