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ISSN 1672-9145 Acta Biochim Biophys Sin 2004, 36(11): 773–779 CN 31-1940/Q

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Accumulation of Pathogenesis-related Type-5 Like Proteins in Phytoplasma-infected Garland Chrysanthemum Chrysanthemum coronarium

Bo-Xiong ZHONG* and Yan-Wei SHEN

Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China

Abstract Soluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble proteins, determined by Edman degradation, shared high sequence similarities with those of pathogenesis-related type-5 (PR-5) proteins such as tobacco thaumatin-like protein. Accumulation of these six proteins was also found in garland chrysanthemum plants infected by other phytoplasmas. These results demonstrate that phytoplasmal infection induces the accumulation of PR-5 like proteins in garland chrysanthemum plants.

Key words phytoplasma; garland chrysanthemum Chrysanthemum coronarium; pathogenesis-related proteins; thaumatin; osmotin

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Received: August 6, 2004 Accepted: September 27, 2004

*Corresponding author: Tel, 86-571-86971302; Fax, 86-571-86945417; E-mail, [email protected]