http://www.abbs.info
E-mail:abbs@sibs.ac.cn
ISSN 0582-9879 Acta Biochim et Biophysica Sinica 2004, 36(4):309-313 CN
31-1300/Q
Expression of a Modified Cry1Ie Gene in E. coli and in Transgenic Tobacco Confers Resistance to Corn Borer
Yun-Jun LIU, Fu-Ping SONG1, Kang-Lai HE1, Yuan YUAN,
Xiao-Xia ZHANG, Peng GAO, Jian-Hua WANG, and Guo-Ying WANG *
( State Key Laboratory for Agrobiotechnology, National Center for Maize
Improvement, China Agricultural University, Beijing 100094, China;
1 State Key Laboratory for Biology of Plant Diseases and Insect
Pests, Institute of Plant Protection, CAAS, Beijing 100094, China )
Abstract The wild-type Cry1Ie gene from Bacillus thuringiensis was modified for its efficient expression in transgenic plants. Modified Cry1Ie gene (designated as Cry1Iem) was cloned into prokaryotic expression vector pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays using crude expression products in E. coli revealed that Cry1Iem protein had a similar toxicity to corn borer as wild-type Cry1Ie. Cry1Iem gene was then inserted downstream of the maize ubiquitin-1 promoter in plant expression vector p3301. Transgenic tobacco plants carrying Cry1Iem showed insecticidal activity against corn borer.
Key words Bacillus thuringiensis; Cry1Iem; transgenic tobacco; insect-resistance
-----------------
Received:
December 29, 2003 Accepted: February 26, 2004
This work was supported by the grants from the High Technology Research and
Development Program of China (2001AA212051) and State Plant Transformation and
Industrialization Program (JY03-B-15)
*Corresponding author: Tel, 86-10-62892577; Fax, 86-10-62892012; E-mail, gywang@cau.edu.cn