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ISSN 0582-9879 Acta Biochim et Biophysica Sinica 2004, 36(5):371-374 CN 31-1300/Q

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The Fate of Mitochondria in Ibex-hirus Reconstructed Early Embryos

Yan JIANG, Shu-Zhen LIU, Yan-Ling ZHANG, Man-Xi JIANG, Qing-Yuan SUN, and Da-Yuan CHEN*
( State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Sciences, Beijing 100080, China )

Abstract Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages.

Key words Capra ibex; cloned embryos; nuclear transfer; mitochondria; PCR

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Received: December 24, 2003 Accepted: March 25, 2004
This work was supported by a grant from the Special Funds for Major Basic Research Project of China (G 20000161)
*Corresponding author: Tel, 86-10-62560528; Fax, 86-10-62565689; E-mail, chendy@panda.ioz.ac.cn