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ISSN
0582-9879 Acta Biochim et Biophysica Sinica 2004, 36(6): 405-411 CN 31-1300/Q
Transcriptional Regulation of Urokinase Receptor in High- (95D) and Low-metastatic (95C) Human Lung Cancer Cells
Chun-Yang LI, Li TAN, Guo-Jing ZHANG, Ping LI, Chang TONG, Jing FAN, and Yun-Song ZHU*
Key Laboratory, the Education Ministry, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Abstract To study the
transcriptional regulation of urokinase receptor (uPAR) in high- (95D) and
low-metastatic (95C) human lung cancer cells, we performed PCR to amplify 2238
bp uPAR promoter from 95C and 95D cells. According to the results of
sequencing, five different bases are found in uPAR promoter between 95C and 95D
cells. The results of luciferase activity assay showed that these differences
have no significant effect on the uPAR promoter activity. Based on a normal
uPAR promoter, progressive truncated mutants were constructed. The transient
transfection/reporter assay showed that the promoter region from -136 to +9 may interact with relevant
nuclear factors, which result in different levels of uPAR expression between
95C and 95D cells.
Key words uPAR; promoter;
transcriptional regulation
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Received: March 18, 2004 Accepted: May 9, 2004
The work was supported by a grant from the National Natural Science Foundation of China (No. 30170398)
*Corresponding author: Tel, 86-21-54237278; E-mail, [email protected]