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ISSN 1672-9145 Acta Biochim Biophys Sin 2004, 36(9): 589-596 CN 31-1940/Q

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Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig

Jian-Zhong YI, Ming-Qiu LIU, Cai-Zhu ZHU¹, Qiang ZHANG, Zu-Tian SHENG², Qing-Yun DU², Wei-Yao YAN, and Zhao-Xin ZHENG*

State Key Laboratory of Genetics Engineering, Life Science School, Fudan University, Shanghai 200433, China;

¹The Veterinary Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;

²The Virus Research Institute of Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Abstract In this study, two DNA fragments encoding amino acid (141-160)-(21-40)-(141-160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138-160)-(21-40)-(138-160) of the serotype A FMDV were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A. The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine-IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses. FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 mg of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.

Key words foot-and-mouth disease; FMDV serotype O; FMDV serotype A; VP1 protein; bivalent vaccine; guinea pig

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Received: May 11, 2004 Accepted: July 12, 2004

This study was supported by a grant from the National High Technology Research and Development Program of China (No. 2001AA213071)

*Corresponding author: Tel, 86-21-65642504; Fax, 86-21-65642504; E-mail, [email protected]