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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(9): 618–622
CN 31-1940/Q
Trichostatin A Extends the Lifespan of Drosophila melanogaster by Elevating hsp22 Expression
Dan TAO#, Jun LU#, Hui SUN, Yan-Mei ZHAO, Zhi-Gen YUAN, Xiao-Xue LI, and Bai-Qu HUANG*
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China
Abstract The level of acetylation of histones in nucleosomes is related to the longevity of yeast and animals. However, the mechanisms by which acetylation and deacetylation affect longevity remain unclear. In present study, we investigated the influence of histone acetylation modification on the expression of hsp22 gene and the lifespan in Drosophila melanogaster using histone deacetylase (HDAC) inhibitor Trichostatin A (TSA). The results showed that TSA could extend the lifespan of Drosophila melanogaster. Furthermore, TSA significantly promoted the hsp22 gene transcription, and affected the chromatin morphology at the locus of hsp22 gene along the polytene chromosome. Present data implicate that TSA may affect the lifespan of Drosophila through changing the level of histone acetylation and influencing the expression of hsp22 gene that is related to aging.
Key words histone acetylation; histone deacetylase inhibitor; Drosophila melanogaster; hsp22; lifespan
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Received: Match 26, 2004 Accepted: July 2, 2004
This work was supported by the grants from the National Natural Science Foundation of China (30370316) and the Major State Basic Research Project of China (G1999053902)
#These authors contributed equally to this work
*Corresponding author: Tel, 86-431-5099768; Fax, 86-431-5681833; E-mail, [email protected]