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ISSN 1672-9145 Acta Biochim Biophys Sin 2004, 36(9): 623–628 CN 31-1940/Q

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Interaction between Plasminogen Activator Inhibitor Type-2 and Pre-mRNA Processing Factor 8

Jing FAN, Yu-Qing ZHANG, Ping LI, Chang TONG, Li TAN, and Yun-Song ZHU*

Department of Molecular Genetics, Shanghai Medical School of Fudan University, Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai 200032, China

Abstract The plasminogen activator inhibitor type-2 (PAI-2) dependent apoptosis protection is due to the 33 amino acids fragment located between helix C and D of PAI-2, this fragment may interact with some unknown intracellular proteins. In this study we used the fragment between helix C and D of PAI-2 as a bait to perform a yeast two-hybrid screen using a cDNA library constructed with HeLa cells during apoptosis, and retrieved a clone encoding 94 amino acid residues of C-terminus of pre-mRNA processing factor 8 (PRPF8). Co-immunoprecipitation experiments confirmed that PAI-2 could interact with PRPF8 in vivo. PAI-2 could bind PRPF8 C-terminal in both the inside and outside of nuclear. These results suggested that the interaction between these two proteins might not be involved in the apoptosis process.

Key words plasminogen activator inhibitor type-2; pre-mRNA processing factor 8 (PRPF8); yeast two-hybrid system; U5

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Received: June 9, 2004 Accepted: July 29, 2004

This work was supported by a grant from the National Natural Science Foundation of China (No. 30070412)

*Corresponding author: Tel, 96-21-54237278; E-mail, [email protected]