http://www.abbs.info E-mail: [email protected]

ISSN 1672-9145 Acta Biochim Biophys Sin 2005, 37(1): 55–60 CN 31-1940/Q

---

RNA-binding Domain of the Key Structural Protein P7 for the Rice dwarf virus Particle Assembly

Bo-Xiong ZHONG^1,2*, Yan-Wei SHEN¹, and Toshihiro OMURA²

¹Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China;

²National Agricultural Research Center, Tsukuba, Ibaraki 305, Japan

Abstract The Rice dwarf virus (RDV) P7 structural protein is the key protein in the RDV particle assembly. The P7 protein was digested partially or completely by Staphylococcus aureus V8 protease and/or Pseudomonas fragi Asp-N protease. The molecular mass and the N-terminal amino acid sequence of the polypeptide fragments of the P7 protein were determined by SDS-PAGE and the Edman degradation method, respectively. Then the polypeptides were located in the deduced amino acid sequence of the RDV P7 protein based on the nucleotide sequence information, with the knowledge of the specific cleavage sites of the Staphylococcus aureus V8 and Pseudomonas fragi Asp-N protease, and the two RNA-binding domains in the P7 protein were identified. Domain 1 was located in the residue 128249 containing 122 amino acids and domain 2 was located in the residue 325355 containing 31 amino acids. Thus, these two domains may play an important role in the virus particle assembly by contributing to the packaging of viral dsRNAs inside the particles. The two domains may be novel RNA-binding domains, because no amino acid sequences highly similar to the conservative sequences of known dsRNA-binding domains reported so far. The similarity between the motif of domain 1 and the motif of the DNA-binding protein suggests that the DNA-binding activity of the RDV P7 protein may be due to this sequence. The similarity between the motif of domain 1 and the motif of the RNA polymerase domain suggests that the P7 protein may also play a role in RNA synthesis, besides its function in the assembly and subsequent packaging of viral dsRNA into core particles.

Key words RDV; RNA-binding protein; protein functional domain; virion assembly; RNA polymerase

-----------------

Received: September 24, 2004 Accepted: November 10, 2004

*Corresponding author: Tel, 86-571-86971302; Fax, 86-571-86971302; E-mail, [email protected]