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ISSN 1672-9145                                                Acta Biochim Biophys Sin 2005, 37(2): 97–106                                                   CN 31-1940/Q

Proteomics-based Identification of Proteins with Altered Expression Induced by 12-O-Tetradecanoylphorbol 13-acetate in Nasopharyngeal Carcinoma CNE2 Cells

 

 

Pei-Zhou JIANG1,2, Ming GAN3, Hua HUANG1, Xin-Ming SHEN1, Shuang WANG1, and Kai-Tai YAO1*

 

1Cancer Research Institute, Southern Medical University, Guangzhou 510515, China;

2Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China;

3Department of Parasitology, Zhongshan Medical College, SUN Yat-sen University, Guangzhou 510080, China

 

Abstract        Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence in Southern China and South-East Asia. Etiology studies indicate that chemical carcinogen promoters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA), are important factors causing NPC development. However, the mechanism of the TPA effect on NPC remains unclear. In the present study, cells from a poorly differentiated squamous cell carcinoma NPC cell line, CNE2, were stimulated by TPA and proteomics technology was carried out to find protein discrepancies between control and TPA-treated cells. Results revealed that TPA treatment in CNE2 cells could upregulate the expression of “triosephosphate isomerase” and “14-3-3 protein sigma” and downregulate the expression of “reticulocalbin 1 precursor”, “nucleophosmin”, “mitochondrial matrix protein p1 precursor”, and “stathmin”. The changes in the expression of these genes suggested that TPA induced CNE2 cells to antiproliferation and to apoptosis, which was confirmed by subsequent apoptosis detection. Therefore, the effects of TPA on nasopharyngeal carcinoma cells were distinct from the effects on primary epithelial cells and we suggest reasons for these differences.

 

Key words        12-O-tetradecanoyl-phorbol-13-acetate; nasopharyngeal carcinoma; proteomics; apoptosis

 

 

 

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Received: November 1, 2004        Accepted: December 23, 2004

*Corresponding author: Tel/Fax, 86-20-61648225; Fax, 86-20-61648225; E-mail, [email protected]