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ISSN
1672-9145
Acta Biochim Biophys Sin 2005, 37(3):
186–191
CN 31-1940/Q
Isolation, Sequence Analysis and Expression Profile of a Novel Swine
Gene Differentially Expressed in the Longissimus Dorsi Muscle Tissues from
Landrace×Large White Cross-combination
Yong-Gang LIU, Yuan-Zhu XIONG*,
and Chang-Yan DENG
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture,
Huazhong Agricultural University,
Abstract The
mRNA differential display technique was performed to investigate the
differences in gene expression in the Longissimus dorsi muscle tissues from
Landrace×Large White cross-combination. One novel gene that was differentially
expressed was identified using semi-quantitative reverse
transcriptase-polymerase chain reaction (RT-PCR) and its complete cDNA sequence
was obtained using the rapid amplification of cDNA ends (RACE) method. The
nucleotide sequence of the gene is not homologous to any of the known porcine
genes. The sequence prediction analysis revealed that the open reading frame of
this gene encodes a protein of 260 amino acids that contains the putative
conserved domain of the carbonic anhydrase, and this protein has high homology
with the carbonic anhydrase III (CA-III) of four species mouse (91%), horse
(91%), rat (89%) and human (86%)––so that it can be defined as swine carbonic
anhydrase III. The phylogenetic tree analysis revealed that the swine CA-III
has a closer genetic relationship with the horse CA-III than with those of
mouse, rat and human. The tissue expression analysis indicated that the swine
CA-III gene is generally expressed in most tissues. Our experiment is the first
to establish the primary foundation for further research on the swine CA-III
gene.
Key words mRNA
differential display; rapid amplification of cDNA ends (RACE); novel gene;
carbonic anhydrase III (CA-III)
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Received: October 18, 2004
Accepted: January 22, 2005
This work is supported by a grant from the National Basic Research
Program of China (No. G2000016105).
*Corresponding author: Tel, 86-27-87287390; Fax, 86-27-87394184;
E-mail, [email protected]