http://www.abbs.info E-mail: [email protected]
ISSN
1672-9145
Acta Biochim Biophys Sin
2005, 37(4): 241–247 CN 31-1940/Q
Identification of the Alternative Promoters of the KChIP4
Subfamily
Xiao-Yun DENG, Fang CAI, Kun
XIA*, Qian PAN, Zhi-Gao LONG, Ling-Qian WU, De-Sheng LIANG, He-Ping DAI,
Zhuo-Hua ZHANG, and Jia-Hui XIA
National Laboratory of Medical Genetics, Central South University,
Changsha 410078, China
Abstract The subfamily of
voltage-dependent potassium (Kv) channel interacting protein 4 (KChIP4) is made
up of the auxiliary interacting protein of voltage-dependent potassium channels.
In this study, the structure of four splicing variants of the human KChIP4
gene was analyzed. Three of the four isoforms of the KChIP4 gene, KChIP4.1,
KChIP4.2 and KChIP4.4, were amplified from mouse and human fetal
brain tissues by reverse transcription-polymerase chain reaction and then
identified. Based on the bioinformatics analysis of the genomic sequences of
the gene, we cloned and characterized two promoter fragments from the KChIP4
gene. One was a 325 bp fragment upstream of the 5' end of the KChIP4.1
mRNA sequence and the other was an 818 bp fragment located immediately at the 5'
end of the KChIP4.4 variant. Both of them can initiate the transcription
of the reporter gene in HT1080 cells and Sprague-Dawley (SD) rat fetal brain
neurons, and they contain C+G islands, except typical TATA boxes and CAAT boxes. This shows that
the KChIP4 gene expression is regulated by an alternative promoter.
Key words splicing variant;
alternative promoter; transcriptional regulation
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Received: December 14, 2004 Accepted: February 25,
2005
This work was supported by the National High Technology Research and
Development Program of China (2002BA711A07-08), the Major State Basic Research
Development Program of China (2004CB518601), and National Natural Science
Foundation of China (30300101, 30123006, and 30400226)
*Corresponding author: Tel, 86-731-4472093; Fax, 86-731-4478152; E-mail, [email protected]