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ISSN 1672-9145                                                Acta Biochim Biophys Sin 2005, 37(5): 355–361                                                   CN 31-1940/Q

Possible Effect of 30K Proteins in Embryonic Development of Silkworm Bombyx mori

 

 

Bo-Xiong ZHONG1*, Jian-Ke LI1,2, Jian-Rong LIN3, Jian-She LIANG4, Song-Kun SU1, Hai-Sheng XU1, Hai-Yan YAN1, Ping-Bo ZHANG5, and Hiroshi FUJII5

 

1Laboratory of Molecular Biology of Silkworm and Honeybee, Department of Bioresource Science, College of Animal Science,

Zhejiang University, Hangzhou 310029, China; 2Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, China;

3Department of Sericulture, South China Agricultural University, Guangzhou 510642, China;

4Biochemistry Co. Ltd. of Zhejiang University, Hangzhou 310029, China;

5Faculty of Agriculture, Kyushu University, Fukuoka 812-81, Japan

 

Abstract        The silkworm Bombyx mori possesses a 30K protein family of 3×104 Da, the biological functions of which have not been fully identified. The relationship between the 30K protein family and the embryonic development of temperature sensitive sex-linked mutant strain of silkworm was investigated by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The results show that protein spots 1–5 of the 30K protein family, mainly existing in normal strain, are possibly related to embryonic development. The early consumption of a 30K protein named 6G1-30K-1 and the accumulation of 30K proteins named 6G1-30K-3 and 6G1-30K-4 are likely caused by the destruction of physiological balance in normal embryonic development, which may lead to lower hatchability of the temperature sensitive strain. The results suggest that reasonable metabolism of 30K proteins is a prerequisite for the embryo’s normal development.

 

Key words        silkworm Bombyx mori; 30K protein; embryonic development; 2D-PAGE; MALDI-TOF MS

 

 

 

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Received: January 5, 2005        Accepted: March 17, 2005

This work was supported by grants from the National Natural Science­ Foundation of China (No. 30271004) and the Natural Science Foundation­ of Guangdong Province (No. 021022)

*Corresponding author: Tel/Fax, 86-571-86971302; E-mail, [email protected]