Properties of Recombinant Aeromonas punctata Prolyl Endopeptidase LI Min, SHEN Guo-Xiang and CHEN Chang-Qing ( Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai 200233, China ) WANG De-Bao ( State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemsitry, Chinese Academy of Sciences, Shanghai 200031, China ) Abstract The properties of recombinant Aeromonas […]
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Purification and Characterization of an Alkaline Lipase from Penicillium cyclopium PG37 WU Min-Chen, WANG Shu, HUANG Wei-Da and SUN Chong-Rong ( Department of Biochemistry, Fudan University, Shanghai 200433, China ) Abstract An extracellular, novel alkaline lipase produced by Penicillium cyclopium PG37 was purified by centrifugation, ammonium sulfate precipitation, and phenyl-Sepharose CL-4B, DEAE Sepharose fast flow […]
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Expression of E
Expression of E-selectin in Endothelial Cells Effects of Wild Type p53 Gene LIN Yong, LI Jian1, GAO Cun-Ji2 and WANG Zhong2 ( Department of Pharmacology, Capital University of Medical Sciences, Beijing 100054, China； 1Department of Biochemistry, Institute of Gerontology, Ministry of Health, Beijing 100005, China； 2Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, […]
Immunomodulated Signaling in Macrophages:Regulation of the MAPK Signaling Pathways by PKA and PKC LIN Min-Qun and CHANG Zong-Liang ( Shanghai Institute of cell Biochemistry， Chinese Academy of Sciences，Shanghai 200031，China ) Abstract Previous experimental investigation indicated that immuno-suppressor activities of suppressor macrophages on T，B lymphocytes and NK cells could be prevented by treatment with LPS，but the […]
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Expression of a Gene for Single
Expression of a Gene for Single-chain Antibody to Carcinoembryonic Antigen in Bombyx mori cells and Its Larvae LI Biao1,2, ZHU Cheng-Mo1, YANG Wei-Dong1,2 and WU Xiang-Fu2 ( 1Rui Jin Hospital, Shanghai No.2 Medical University, Shanghai 200025, China； 2Shanghai Institute of Biochemistry, the Chinese Academia of Sciences, Shanghai 200031, China ) Abstract Recombinant Bm-BacScFv virus which […]
The Relationship between Yeast Coexpressed Gene Clusters and Their Upstream cis-acting Elements XIE Tao, SHENG Quan-Hu and DING Da-Fu ( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China ) Abstract DNA microarrays bring biology a new approach to study functions of genes and genomes from their expression pattern on a genomic […]
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Overexpressed Human TNF Receptor
Overexpressed Human TNF Receptor-75 Can Independently Mediate hTNFα-induced Cytotoxicity in BHK-21 Cells FANG Jia, WANG De-Bao1 and CHEN Chang-qing ( Shanghai Research Center of Biotechnology, the Chinese Academy of Sciences, Shanghai 200233, China； 1State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China ) DUCH Mogens Ryttergaard […]
Molecular Cloning and Expression of a New A Chain of β-Bungarotoxin QIAN You-Cun, FAN Chun-Yang, LIU Xiao-Long1, HU Tai-Shan, YANG Sheng-Li and GONG Yi ( Shanghai Research Center of Biotechnology, CAS, Shanghai 200233, China； 1Shanghai Institute of Biochemistry, CAS, Shanghai 200031, China ) Abstract The cDNA encoding a new A chain of β-bungarotoxin was cloned […]
Analysis of Peptide Mapping，Glycosylated Site and Glycan Structure in Ribonuclease B by Liquid Chromatography-Electrospray Mass Spectrometry ZENG Rong，SHAO Xiao-Xia，WANG ke-Yi and XIA Qi-Chang ( Shanghai Institute of Biochemistry， Chinese Academy of Sciences， Shanghai 200031， China ) Abstract Liquid chromatography-electrospray mass spectrometry was utilized to analyze peptide mapping of aglycoprotein，ribonuclease B，to obtain its primary structure.The glycosylated […]
Cloning and Expression of Human Manganese-superoxide Dismutase cDNA SHI Hui-Juan1，HE Hua-Jun，WEI Dong-Zhi and YUAN Qin-Sheng ( Research Institute of Biochemistry，State Key Laboratory of Bioreactor Engineering，East China University of Science and Technology，Shanghai 200237，China ) CHEN Zhe-Yu，LI Ming-Feng and WU Xiang-Fu ( 1Shanghai Institute of Biochemistry，the Chinese Academy of Sciences，Shanghai 200031，China ) Abstract The cDNA encoding human […]