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Abstract |
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Vol 48 No. 3: 290-297 |
[PDF] [Full Text] |
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MicroRNA-195a-5p inhibits mouse medullary thymic epithelial cells proliferation by directly targeting Smad7 |
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Dongguang Guo,
Yaqiong Ye,
Junjie Qi,
Lifeng Xu,
Lihua Zhang,
Xiaotong Tan,
Zhigang Tan,
Xiaofang Yu,
Yuan Zhang,
Yongjiang Ma and
Yugu Li*
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College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China |
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Abstract MiR-195 has been implicated in inhibiting cell proliferation in different types of tumors. Whether it contributes to the process of thymic epithelial cells (TECs) proliferation remains unclear. In this study, we found that miR-195a-5p was highly up-regulated in the TECs isolated from the aging mice. Further experiments showed that miR-195a-5p mimic transfection inhibited the proliferation of mouse medullary thymic epithelial cell line 1 (MTEC1), whereas the transfection of miR-195a-5p inhibitor in MTEC1 had the opposite effect. In addition, miR-195a-5p had no obvious effect on MTEC1 apoptosis. Furthermore, Smad7, a negative regulator of transforming growth factor β pathway, was confirmed as a direct target of miR-195a-5p by luciferase assays. Taken together, our results indicate that miR-195a-5p inhibits MTEC1 proliferation, at least in part, via down-regulation of Smad7. |
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Keywords miR-195a-5p; mouse thymic epithelial cell; cell proliferation; Smad7 |
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Received 2015-8-13
Accepted 2015-11-2 |
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Funding This work was supported by the grants from the National Natural Science Foundation of China (Nos. 31272519 and 31572475). |
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* Correspondence address Tel/Fax: +86-20-85283226; E-mail: [email protected]; [email protected] |
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