Mailing Address:
Department of Biochemistry
Dartmouth Medical School
Hanover, NH 03755
Tel. 603-650-1622
Fax. 603-650-1128
e-mail: [email protected]
Chang Laboratory Website: http://www.Dartmouth.edu/~tychang
Date of Birth: April 8, 1945
Education:
B.S. in Chemistry, National Taiwan University, Taiwan, 1967
Ph.D. in Biochemistry (with Mary Ellen Jones), University of North
Carolina, Chapel Hill, North Carolina, 1968-1973
Postdoctoral Fellow (with Roy Vagelos), Department of Biological
Chemistry, Washington University School of Medicine, St. Louis, Missouri,
1973-1975
Postdoctoral Fellow (with Roy Vagelos), Merck, Sharp & Dohme
Research Laboratories, Rahway, New Jersey, 1975-1976
Professional Experience:
2000-present Professor and Chair,
Department of Biochemistry, Dartmouth Medical School
1988-2000 Professor, Department of
Biochemistry,
Dartmouth Medical School
1982-1988 Associate Professor, Department
of Biochemistry, Dartmouth Medical School
1976-1982 Assistant Professor, Department
of Biochemistry, Dartmouth Medical School
Membership:
American Society of Biological Chemists and Molecular Biologists,
1981-present
American Society of Cell Biologists, 1999-present
Extramural Activities:
Member of Editorial Board, Journal of Biological Chemistry
(1995-2000)
Member of Editorial Board, Journal of Biological Chemistry
(2001-2006)
Visiting Professor, Kumamoto University Medical School, Kumamoto,
Japan (1995-1997; 1999-2000)
Member, American Heart Association National Review Committee on
Lipids and Lipoproteins (1998-2002)
Visiting Professor, University of Hong Kong (2003)
Honors:
NIH Research Career Development Award (1982-1987)
NIH MERIT Award (1994-2004)
Current Research Support:
1. NIH R37 HL36709 (1994-2004) Cell
Mutants Defective in Cholesterol Ester Formation
2. NIH RO1 HL60306 (2003-2007)
Structure-Function Analyses of ACAT
Invited Speaker at Selected National and
International Meetings, 1987-2003:
- American Society of Biological
Chemists Satellite Symposium on Cellular Cholesterol Metabolism (1987),
Chair: Dr. Michael Sinensky - Aspen Cholesterol Conference (1987),
Chair: Dr. Roger Davis - Gordon Research Conference on
Atherosclerosis (1989), Chairs: Drs. Thomas Innerarity and Alan Fogelman - International Symposium on Drugs
Affecting Lipid Metabolism, Houston, Texas (1989), Chair: Dr. Antonio
Gotto - Aspen Cholesterol Conference (1991),
Chairs: Drs. Daniel Steinberg and Donald Small - Japanese Research Conference on
Cholesterol, Shirahama, Japan (1991), Chair: Dr. Seikoh Horiuchi - International Conference on
Atherosclerosis and Thrombosis, Tokyo, Japan (1992), Chair: Dr. Akira
Yamamoto - Aspen Cholesterol Conference (1993),
Chair: Dr. Larry Rudel - Xth International Symposium on
Atherosclerosis, Montreal, Canada (1994), Chair: Dr. Jean Davignon - Aspen Cholesterol Conference (1995),
Chair: Dr. John Dietschy - American Heart Association National
Meeting (1996), State of the Art Speaker in Lipid Metabolism Session - FASEB Summer Research Conference on
Intestinal Lipid Digestion (1997), Chair: Dr. David Hui - International Satellite Conference on
Cellular Cholesterol Metabolism, San Francisco, California (1997), Chair:
Dr. Sandra Erickson - Gordon Research Conference on Lipid
Metabolism (1998), Chair: Dr. David Williams - International Symposium on
Lipoprotein Metabolism and Atherogenesis, Kyoto, Japan (1998), Chair: Dr.
Toru Kita - International Conference on the NPC
Lesion and the Role of Intracellular Cholesterol Trafficking in Human
Disease, Washington DC (1999), Chair: Dr. Peter Pentchev - FASEB Summer Research Conference on
Intestinal Lipid Digestion (2000), Chair: Dr. Jeff Field - ZMBH Forum (2001), “Genes,
Proteins, and Brain Disease”, Heidelberg, Germany. Organizer: Tobias
Hartmann - Gordon Research Conference on
Molecular and Cell Biology of Lipids (2001), Chair: Charles Rock - Gordon Research Conference on
Lipoprotein Metabolism (2002), Session Chair and Speaker, Chair: Mike
Phillips. - American Heart Association National
Meeting (2002), Session Chair and Speaker
Publications in Peer-Reviewed Journals:
- Chang, T.Y. and Jones, M.E. Aspartate
Transcarbamylase from Streptococcus faecalis. Purification,
Properties and Nature of an Allosteric Activator Site. Biochemistry
13, 629 (1974) - Chang, T.Y. and Jones, M.E. Aspartate
Transcarbamylase from Streptococcus faecalis. Steady-State Kinetic
Analysis. Biochemistry 13, 637 (1974) - Chang, T.Y. and Jones, M.E. Aspartate
Transcarbamylase from Streptococcus faecalis. Reverse Reaction and
Binding Studies. Biochemistry 13, 646 (1974) - Chang, T.Y. and Vagelos, P.R. Isolation and
Characterization of an Unsaturated Fatty Acid Requiring Mutant of Cultured
Mammalian Cells. Proc. Natl. Acad. Sci. U.S.A. 73, 24 (1976)
- Chang, T.Y., Telakowski, C., Vanden Heuvel, W.,
Alberts, A.W., and Vagelos, P.R. Isolation and Partial Characterization of
a Cholesterol-Requiring Mutant of Chinese Hamster Ovary Cells. Proc.
Natl. Acad. Sci. U.S.A. 74, 833 (1977) - Nelson, J.A., Czarny, M.R., Spencer,
T.A., Limanek, J.S., McCrae, K.R., and Chang, T.Y. A Novel
Inhibitor of Steroid Biosynthesis. J. Am. Chem. Soc. 100,
4900 (1978) - Limanek, J.S., Chin, J., and Chang,
T.Y. A Mammalian Cell Mutant Requiring Cholesterol and Unsaturated
Fatty Acid for Growth. Proc. Natl. Acad. Sci. U.S.A. 75,
5452 (1978) - Chang, T.Y., Schiavoni, E.S., Jr., McCrae, K.R.,
Nelson, J.A., and Spencer, T.A. Inhibition of Cholesterol Biosynthesis in
Chinese Hamster Ovary Cells by 4,4,10-Trimethyltransdecal-3b -o1. A Specific 2,3-Oxidosqualene
Cyclase Inhibitor. J. Biol. Chem. 254, 11258 (1979) - Chang, T.Y. and Limanek, J.S. Regulation of
Cytosolic Acetoacetyl CoA Thiolase, 3-Hydroxy-3-Methylglutaryl CoA
Synthase, 3-Hydroxy-3-Methylglutaryl CoA Reductase, and Mevalonate Kinase
by Low Density Lipoprotein and by 25-Hydroxycholesterol in Chinese Hamster
Ovary Cells. J. Biol. Chem. 255, 7787 (1980) - Chang, T.Y., Limanek, J.S., and Chang, C.C.Y.
Evidence Indicating that Inactivation of 3-Hydroxy-3-Methylglutaryl
Coenzyme A Reductase by Low Density Lipoprotein or by
25-Hydroxycholesterol Requires Mediator Protein(s) with Rapid Turnover
Rate. J. Biol. Chem. 256, 6174 (1981) - Chin, J. and Chang, T.Y.
Evidence for Coordinate Expression of 3-Hydroxy-3-Methylglutaryl Coenzyme
A. Reductase and Low Density Lipoprotein Binding Activity. J. Biol.
Chem. 256, 6304 (1981) - Chang, T.Y., Limanek, J.S., and Chang, C.C.Y. A
Simple and Efficient Procedure for the Rapid Homogenization of Cultured
Animal Cells Grown in Monolayer. Anal. Biochem. 116, 298
(1981) - Berry, D.J. and Chang, T.Y.
Further Characterization of a Chinese Hamster Ovary Cell Mutant Defective
in Lanosterol Demethylation. Biochemistry 21, 573 (1982) - Doolittle, G.M. and Chang, T.Y. Solubilization,
Partial Purification and Reconstitution in Phosphatidylcholine-Cholesterol
Liposomes of Acyl-CoA:Cholesterol Acyltransferase. Biochemistry 21,
674 (1982) - Chin, J. and Chang, T.Y.
Further Characterization of a Chinese Hamster Ovary Cell Mutant Requiring
Cholesterol and Unsaturated Fatty Acid for Growth. Biochemistry 21,
3196 (1982) - Chang, T.Y. and Chang, C.C.Y. Revertants of
Chinese Hamster Ovary Cell Mutant Resistant to Suppression by Analog of
Cholesterol-Isolation and Partial Characterization. Biochemistry 21,
5316 (1982) - Doolittle, G.M. and Chang, T.Y.
Acyl-CoA:Cholesterol Acyltransferase in Chinese Hamster Ovary Cells:
Enzyme Activity Determined after Reconstitution in
Phospholipid-Cholesterol Liposomes. Biochim. Biophys. Acta 713,
529 (1982) - Chang, C.C.Y., Doolittle, G.M., and Chang,
T.Y. Cycloheximide-Sensitivity in Regulation of Acyl-CoA:Cholesterol
Acyltransferase in Chinese Hamster Ovary Cells. I. Effect of Exogenous
Sterols. Biochemistry 25, 1693 (1986) - Chang, C.C.Y. and Chang, T.Y.
Cycloheximide-Sensitivity in Regulation of Acyl-CoA:Cholesterol
Acyltransferase in Chinese Hamster Ovary Cells. II. Effect of Endogenous
Sterol Synthesis. Biochemistry 25, 1700 (1986) - Ventimiglia, J., Levesque, M.C, and Chang,
T.Y. Preparation and Characterization of Unilamellar Vesicles from
Cholate-Phospholipid Micelle Treated with Cholestyramine. Anal.
Biochem. 157, 323 (1986) - Cadigan, K., Heider, J., and Chang,
T.Y. Isolation and Characterization of Chinese Hamster Ovary Cell
Mutants Deficient in Acyl-Coenzyme A:Cholesterol Acyltransferase Activity.
J. Biol. Chem. 263, 274 (1988) - Cadigan, K.M. and Chang, T.Y. A
Simple Method for Reconstituting Acyl-CoA:Cholesterol Acyltransferase in
Cholesterol Containing Liposome. J. Lipid Research 29, 1683
(1988) - Cadigan, K.M., Chang, C.C.Y., and Chang,
T.Y. Isolation of Chinese Hamster Ovary Cell Lines Expressing Human
Acyl- Coenzyme A:Cholesterol Acyltransferase Activity. J. Cell Biol.
108, 2201 (1989) - Shi, S.P., Chang, C.C.Y., Gould,
G.W., and Chang, T.Y. Comparing the Unilamellar
Phosphatidylethanolamine Vesicles with the Unilamellar Phosphatidylcholine
Vesicles Produced by Treating Cholate-phospholipid Micelle with
Cholestyramine. Biochim. Biophys. Acta 982, 187 (1989) - Cadigan, K.M., Spillane, D.M., and Chang,
T.Y. Isolation and Characterization of Chinese Hamster Ovary Cell
Mutants Defective in Intracellular Low Density Lipoprotein-Cholesterol
Trafficking. J. Cell Biol. 110, 295 (1990) - Hasan, M.T., Subbaroyan, R., and Chang,
T.Y. High-efficiency Stable Gene Transfection using
Chloroquine-treated CHO cells. Somatic Cell & Mol. Genetics 17,
513 (1991) - Chang, C.C.Y., Huh, H.Y., Cadigan,
K.M., and Chang, T.Y. Molecular Cloning and Functional Expression
of Human Acyl- CoA:Cholesterol Acyltransferase cDNA in Mutant Chinese
Hamster Ovary Cells. J. Biol. Chem. 268, 20747 (1993) - Hasan, M.T., Chang, C.C.Y., and Chang,
T.Y. Somatic Cell Genetic and Biochemical Characterization of Cell
Lines Resulting from Human Genomic DNA Transfection of Chinese Hamster
Ovary Cell Mutants Defective in Sterol-Dependent Activation of Sterol
Synthesis and LDL Receptor Expression. Somatic Cell & Mol. Genetics
20, 183 (1994) - Chang, C.C.Y., Nutile-McMenemy, N.,
Noll, W., Lindsay, E.A., Baldini, A., Chang, W., and Chang, T.Y.
Localization of Acyl-Coenzyme A:Cholesterol Acyltransferase Gene to Human
Chromosome 1q-25. Somatic Cell & Mol. Genetics 20, 71
(1994) - Hasan, M.T. and Chang, T.Y.
Somatic Cell Genetic Analysis of Two Classes of CHO Cell Mutants
Expressing Opposite Phenotypes in Sterol-Dependent Regulation of
Cholesterol Metabolism. Somatic Cell & Mol. Genetics 20,
481 (1994) - Spillane, D.M., Reagan, J.W.,
Kennedy, N.J., Schneider, D.L., and Chang, T.Y. Translocation of
Both Lysosomal LDL-derived Cholesterol and Plasma Membrane Cholesterol to
the Endoplasmic Reticulum for Esterification May Require Common Cellular
Factors Present in the Acidic Compartment(s). Biochim. Biophys. Acta
1254, 283 (1995) - Cheng, D., Chang, C.C.Y., Qu, X-M.,
and Chang, T.Y. Activation of Acyl-Coenzyme A:Cholesterol
Acyltransferase (ACAT) by Cholesterol or by Oxysterol in a Cell-Free
System. J. Biol. Chem. 270, 685 (1995) - Uelmen, P.J., Oka, K., Sullivan, M.,
Chang, C.C.Y., Chang, T.Y., and Chan, L. Tissue-Specific Expression
and Cholesterol Regulation of Acyl-Coenzyme A:Cholesterol Acyltransferase
(ACAT) in Mice: Molecular Cloning of Mouse ACAT cDNA, Chromosomal
Localization, and Regulation of ACAT In Vivo and In Vitro. J.
Biol. Chem. 270, 26192 (1995) - Chang, C.C.Y., Chen, J., Thomas, M.,
Cheng, D., Del Priore, V.A., Newton, R.S., Pape, M.E., and Chang, T.Y.
Regulation and Immunolocalization of Acyl-Coenzyme A:Cholesterol
Acyltransferase in Mammalian Cells as Studied with Specific Antibodies. J.
Biol. Chem. 270, 29532 (1995) - Matsuda, H., Hakamata, H., Miyazaki,
A., Sakai, M., Chang, C.C.Y., Chang, T.Y., Kobori, S., Shichiri,
M., and Horiuchi, S. Activation of Acyl-Coenzyme A:Cholesterol
Acyltransferase Activity by Cholesterol is Not Due to Altered mRNA Levels
in HepG2 Cells. Biochim. Biophys. Acta 1301, 76 (1996) - Carstea, E.D., Morris, J.A., Coleman,
K.G., Loftus, S.K., Zhang, D., Cummings, C., Gu, J., Rosenfeld, M.A.,
Pavan, W.J., Krizman, D.B., Nagle, J., Polymeropoulos, M.H., Sturley,
S.L., Ioannou, Y.A., Higgins, M.E., Comly, M., Cooney, A., Brown, A.,
Kaneski, C.R., Blanchette-Mackie, E.J., Dwyer, N.K., Neufeld, E.B., Chang,
T.Y., Liscum, L., Strauss, J.F., III, Ohno, K., Zeigler, M., Carmi,
R., Sokol, J., Markie, D., O’Neill, R.R., Van Diggelen, O.P., Elleder, M.,
Patterson, M.C., Brady, R.O., Vanier, M.T., Pentchev, P.G., and Tagle,
D.A. The Niemann-Pick C Gene: Homology to Mediators of Cholesterol
Homeostasis. Science 277, 228 (1997) - Rawson, R.B., Zelenski, N.G.,
Nijhawan, D., Ye, J., Sakai, J., Hasan, M.T., Chang, T.Y., Brown,
M.S., and Goldstein, J.L. Complementation Cloning of S2P, a Gene Encoding
a Putative Metalloprotease Required for Intramembrane Cleavage of SREBPs. Molecular
Cell 1, 47 (1997) - Khelef, N., Buton, X., Beatini, N.,
Wang, H., Meiner, V., Chang, T.Y., Farese, R.V. Jr, Maxfield, F.R.,
and Tabas, I. Immunolocalization of Acyl-Coenzyme A:Cholesterol
Acyltransferase in Macrophages. J. Biol. Chem. 273, 11218
(1998) - Li, O., Chang, C., Lee, W., and Chang,
T.Y. Immunodepletion Experiments suggest that Acyl-Coenzyme
A:Cholesterol Acyltransferase 1 Protein is the Major Catalytic Component
in Adult Human Liver, Adrenal Gland, Kidney, and Macrophages, but not in
Human Intestines. J. Lipid Res. 39, 1722 (1998) - Miyazaki, A., Sakashita, N., Lee, O.,
Takahashi,S., Hakamata, H., Morganelli, P., Chang, C.C.Y., and Chang,
T.Y. Expression of Acyl-Coenzyme A:Cholesterol Acyltransferase in
Human Atherosclerotic Lesions and in Cultured Human Monocyte-Macrophages. Arterio.
Thromb. and Vas. Biol. 18, 1568 (1998) - Chang, C.C.Y., Lee, C.Y.G., Chang,
E.T., Cruz, J.C., Levesque, M., and Chang, T.Y. Recombinant
Acyl-CoA:Cholesterol Acyltransferase-1 (ACAT-1) Purified to Essential
Homogeneity Utilizes Cholesterol in Mixed Micelles or in vesicles in a
Highly Cooperative Manner. J. Biol. Chem. 273, 35132 (1998) - Tomita, T., Chang, T.Y.,
Kodama, T., and Iwatsubo, T. ß-APP Gamma-Secretase and SREBP Site 2 Protease
are two Different Enzymes. NeuroReport 9, 911 (1998) - Li, B.L., Li, X.L., Duan, Z., Lee,
O., Lin, S., Ma, Z.M., Chang, C.C.Y., Yang, X.Y., Mohandas, T.K., Noll,
W., Chan, L., and Chang, T.Y. Human ACAT-1 Gene
Organization, and the Evidence that the 4.3 kb mRNA is Produced from two
Different Chromosomes. J. Biol. Chem. 274, 11060 (1999) - Lin, S., Cheng, D., Liu, M.S., Chen,
J., and Chang, T.Y. Human Acyl-CoA:Cholesterol Acyltransferase-1
(ACAT-1) in the Endoplasmic Reticulum Contains Seven Transmembrane
Domains. J. Biol. Chem. 274, 23276 (1999) - Yu, C., Chen, J., Lin, S., Liu, J.,
Chang, C.C.Y., and Chang, T.Y. Human Acyl-CoA:Cholesterol
Acyltransferase-1 (ACAT-1) is a Homotetrameric Enzyme in Intact Cells and In
Vitro. J. Biol. Chem. 274, 36139 (1999) - Sakashita, N., Miyazaki, A., Takeya,
M., Horiuchi, S., Chang, C.C.Y., Chang, T.Y., and Takahashi, K.
Localization of Human Acyl-Coenzyme A:Cholesterol Acyltransferase-1
(ACAT-1) in Macrophages and in Various Tissues. Amer. J. Pathol. 156,
227 (2000) - Cruz, J.C., Sugii, S., Yu, C., and Chang,
T.Y. Role of Niemann-Pick type C1 protein in Intracellular Trafficking
of Low Density Lipoprotein Derived Cholesterol. J. Biol. Chem. 275,
4013 (2000) (chosen as cover page) - Henderson, L.P., Lin, L., Prasad, A.,
Paul, C.A., Chang, T.Y., and Maue, R.A. Embryonic Striatal
Neurons from Niemann-Pick type C Mice Exhibit Defects in Cholesterol
Metabolism and Growth Factor Responsiveness. J. Biol. Chem. 275,
20179 (2000) (chosen as Highlight of Recent Literature by Editor of Science,
289, Number 5477, 14 July 2000) - Chang, C.C.Y., Sakashita, N.,
Ornvold, K., Lee, O., Chang, E., Dong, R., Lin, S., Lee, C.Y.G., Strom,
S., Kashyap, R., Fung, J., Farese, R., Patoiseau, J.F., Delhon, A., and Chang,
T.Y. Immunological Quantitation and Localization of ACAT-1 and ACAT-2
in Human Liver and Small Intestines. J. Biol. Chem. 275,
28083 (2000) - Cruz, J.C., and Chang, T.Y.
Fate of Endogenously Synthesized Cholesterol in Niemann-Pick Type C1
Cells. J. Biol. Chem. 275, 41309 (2000) - Maung, K.K., Miyazaki, A., Nomiyama,
H., Chang, C.C.Y., Chang, T.Y., and Horiuchi, S. Increase in
acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT-1) mRNAs and protein
contents by treating undifferentiated human monocytic THP-1 cells with
1,25-dihydroxyvitamin D3 or with 9-cis retinoic acid. J. Lipid Res. 42,
181 (2001) - Yamazaki, T., Chang, T.Y.,
Haass, C., and Ihara, Y. Accumulation and aggregation of amyloid ß-protein
in late endosomes of Niemann-Pick type C cells. J. Biol. Chem. 276,
4454 (2001) - Yang, J.B., Duan, Z.J., Yao, W., Lee,
O., Yang, L., Yang, X.Y., Sun, X., Chang, C.C.Y., Chang, T.Y., and
Li, B.L. Synergistic Transcriptional Activation of Human ACAT-1 Gene by
IFN-gamma and ATRA in THP-1 Cells. J. Biol. Chem. 276, 20989
(2001) - Song, B.L., Qi, W., Yang, X.Y.,
Chang, C.C.Y., Zhu, J.Q., Chang, T.Y., and Li, B.L. Genomic
Organization of Human ACAT-2 Gene and Cell Type- Specific Expressions of
its Promoter. Biochem. Biophys. Res. Commun. 282, 580 (2001) - Puglielli, L., Konopka, G.,
Pack-Chung, E., Ingano, L.A.M., Berezovska, O., Hyman, B.T., Chang,
T.Y., Tanzi, R.E., and Kovacs, D.M. Acyl-coenzyme A:cholesterol
acyltransferase modulates the generation of the amyloid beta-peptide. Nature
Cell Biol. 3, 905 (2001) - Lu, X.H., Lin, S., Chang, C.C.Y., Chang,
T.Y. Mutant Acyl-coenzyme A:Cholesterol Acyltransferase 1 Devoid of
Cysteine Residues Remains Catalytically Active. J. Biol. Chem. 277,
711 (2002) - Yu, C., Zhang, Y., Lu, X., Chen, J.,
Chang, C.C.Y., and Chang, T.Y. Role of the N-Terminal Hydrophilic
Domain of Acyl-Coenzyme A:Cholesterol Acyltransferase 1 on the Enzyme’s
Quaternary Structure and Catalytic Efficiency. Biochemistry 41,
3762 (2002) - Cruz, J.C., Thomas, M., Wong, E.,
Ohgami, N., Sugii, S., Curphey, T., Chang, C.C.Y., and Chang, T.Y.
Synthesis and Biochemical properties of a New Photoactivatable Cholesterol
Analog 7,7-azocholestanol and its Linoleate Ester in Chinese Hamster Ovary
Cell Lines. J. Lipid Res. 43, 1341 (2002) - Buszczak, M., Lu, X., Segraves, W.A.,
Chang, T.Y., and Cooley, L. Mutations in the midway Gene Disrupt a
Drosophila Acyl Coenzyme A: Diacylglycerol Acyltransferase. Genetics
160, 1511 (2002) - Khan, N., Shen, J., Chang, T.Y.,
Chang, C.C.Y., Fung, P.C.W., Grinberg, O., Demidenko, E., and Swartz, H.
Plasma Membrane Cholesterol: A Possible Barrier to Intracellular Oxygen in
Normal and Mutant CHO Cells Defective in Cholesterol Metabolism. Biochemistry
42(1), 23 (2003) - Zhang, Y., Yu, C., Liu, J., Spencer,
T.A., Chang, C.C.Y., and Chang, T.Y. Cholesterol is superior to
7-ketocholesterol or 7alpha-hydroxycholesterol as an allosteric activator
for acyl-coenzyme A: cholesterol acyltransferase 1. J. Biol. Chem.
In press/Published online Jan. 17 (2003) - Sugii, S., Reid, P.C., Ohgami, N.,
Shimada, Y., Maue, R.A., Ninomiya, H., Ohno-Iwashita, Y., and Chang,
T.Y. Biotinylated theta toxin derivative as a probe to examine
intracellular cholesterol-rich domains in normal and Niemann-pick type C1
cells. J. Lipid Res. In press/Published online Feb. 1 (2003) - Reid, P.C., Sugii, S., and Chang,
T.Y. Trafficking defects in endogenously synthesized cholesterol in
fibroblasts, macrophages, hepatocytes, and glial cells from NPC1 mice. J.
Lipid Res. In press/Published online (2003) - Lin, S., Lu, X., Chang, C.C.Y., and Chang,
T.Y. Human Acyl-Coenzyme A: Cholesterol Acyltransferase 2 (hACAT2)
expressed in Chinese hamster ovary cells: membrane topology and active
site location. Mol. Biol. Cell. In press/Published online (2003) - Sugii, S., Reid, P.C., Ohgami, N.,
Du, H., and Chang, T.Y. Distinct endosomal compartments in early
trafficking of low density lipoprotein-derived cholesterol. J. Biol.
Chem. In revision. (2003) - Sakashita, N., Miyazaki, A., Chang,
C.C.Y., Chang, T.Y., Kiyota, E., Satoh, M., Komohara, Y.,
Morganelli, P., Horiuchi, S., and Takeya, M. Acyl-coenzyme A:cholesterol
acyltransferase 2 (ACAT2) is induced in monocyte-derived macrophages: in
vivo and in vitro studies. Lab. Invest. In revision.
(2003)
Invited Reviews in Books, Review Series,
and Journals:
- Chang, T.Y., Prescott, L.M., and Jones, M.E.
Aspartate Transcarbamylase (Streptococcus faecalis) in Methods
in Enzymology (Hoffee, P.A. and Jones, M.E., Eds), Academic Press, New
York, vol. II, p. 41 (1978) - Chang, T.Y. in The Enzymes,
3rd edition, vol. 16 on Lipid Metabolism (P.D. Boyer, Ed.) Chapter 14 on
“Mammalian HMG-CoA Reductase and Its Regulation”, Academic
Press, 491-521 (1983) - Chang, T.Y. and Doolittle, G.M. in The
Enzymes, 3rd edition, vol. 16 on Lipid Metabolism (P.D. Boyer, Ed.)
Chapter 15 on “Acyl-Coenzyme A:Cholesterol Acyltransferase”,
Academic Press, 523-539 (1983) - Chang, T.Y., Chang, C.C.Y., and Cadigan, K.M.
Structure of Acyl-Coenzyme A:Cholesterol Acyltransferase and its Potential
Relevance to Atherosclerosis. Trends in Cardiovascular Medicine 4,
223 (1994) - Chang, T.Y., Chang, C.C.Y., and Cheng, D.
Acyl-Coenzyme A:Cholesterol Acyltransferase. Ann. Rev. Biochem. 66,
613 (1997) - Chang, T.Y., Hasan, M., Chin, J., Chang, C.C.Y.,
Spillane, D., and Chen, J. Chinese Hamster Ovary Cell Mutants Affecting
Cholesterol Metabolism. Current Opinions in Lipidology 8, 65
(1997) - Chang, T.Y. SREBPs, Membrane Lipid Biosynthesis,
and Fatty Acids. J. Clinical Invest. 100, 1905 (1997) - Chang, T.Y., Chang, C.C.Y., and Lee, O. in Intracellular
Cholesterol Trafficking (Ed. by T.Y. Chang and D. Freeman). Kluwer
Press. Chapter on “The Sterol-Specific Regulation of ACAT-1 and
SREBPs in Mammalian Cells and in Liver”, pp.1-14 (1998) - Chang, T.Y., and Chang, C.C.Y. in Encyclopedia
of Molecular Medicine (Edited by T.E.Creighton), Entry on
“Acyl-Coenzyme A:Cholesterol Acyltransferase”, Wiley & Sons.
(2001) - Chang, T.Y., Chang, C.C.Y., Lu, X.H., and Lin,
S. Catalysis of ACAT may be completed within the plane of the membrane: a
working hypothesis. J. Lipid Research 42, 1933 (2001) - Chang, T.Y., Chang, C.C.Y., Lin, S., Yu, C., Li,
B.L., and Miyazaki, A. Roles of acyl-coenzyme A:cholesterol
acyltransferase-1 and-2. Current Opin. Lipidol. 12, 289
(2001) - Chang, T.Y., Chang, C.C.Y. ACAT as a Target for
Pharmaceutical Intervention of Atherosclerosis. Invited commentary
published on the International Atherosclerosis Society website
(www.athero.org), August 2002.
Books:
1. Intracellular Cholesterol
Trafficking (Ed. by T.Y. Chang and D. Freeman). Kluwer Academic
Press. (1998)
Patents:
Inventor of three patents issued to
Dartmouth College:
1. U.S. Patent # 5,484,727 (Issued
01/16/96)
2. U.S. Patent # 5,834,283 (Issued
11/10/98)
3. U.S. Patent # 5,968,749 (Issued
10/19/99)