Untitled Document

TANG Jian-Guo

CV in Chinese

A, Brief Introduction:

First Name: Jian-Guo

Family Name: Tang

Sex: Male

Birth Date: 1963-02-26

Degree: Ph.D.

Phone: 13701015465 / 10-62987888ext.211

Fax: 10-62980085

Email: [email protected]

Contacting Address:Novozymes China, 14 Xinxi Lu, Shangdi Zone, Haidian District, Beijing 100085, China

Education and Professional Occupations:

1980,9-1985,7 Bachelor of Science in Biochemistry, Jilin University

1985,9-1990,6 Ph.D., Institute of Biophysics, Academia Sinica, supervised by Prof. Chen-Lu Tsou

1990,7-2003,3 College of Life Sciences, Peking University:

Post-doc (1990-1992)

Associate Professor (1992-1996)

Professor (1996- )

Ph.D. Supervisor (1997- )

2003,4-present Manager, Application Development Department, R&D, Novozymes China

Experiences in Abroad:

1994,1-1995,2 Visiting Scholar, University of Cambridge, UK

1999,1-2000,2 Visiting Scholar, UCLA, USA

2000,11-2001,2 Visiting Scholar, University of Melbourne,

Australia

B, Working Experiences:

I have been working in the fields of protein structure/function relationship, gene engineering, protein engineering, gene therapy in the last 15 years with about 50 publications (half of them in western journals) and three patents. The projects include structure/function and folding pathway of insulin family members, structure/function of trypsin and leptin, gene engineering of parathyroid hormone, protein engineering of RGD-containing proteins, gene therapy for diabetes. I have been a group leader with about 10 people since 1993. I have been also responsible for delivering lectures to both graduate and undergraduate students. I am now working on new applications of enzymes in industry.

C, Academic Awards:

1993 Second Award for Natural Science Research, Academia Sinica

1994 Beijing Science and Technology Award for Young People, Beijing Association of Science and Technology

1995 National Science Fund for Outstanding Young People, Natural Science Foundation of China

1995 National Second Award for Natural Science Research, Chinese Government

1997 Second Award of Guang Hua Science and Technology - Ministry level

1998 Award for Excellence in Teaching for Young People, Peking University

1998 National Science and Technology Award for Young People, Association of Science and Technology of China

2001 First Award for Excellent Course on Biochemistry and Molecular Biology in Higher Education, Beijing Municipal Government

2002 Award for Outstanding Faculty, Ministry of Education

D, Services in Academic Society:

2002锟斤拷 Deputy Director of the National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University

2002锟斤拷 Vice President of Beijing Society of Biochemistry and Molecular Biology

1998锟斤拷 Committee Member, Enzyme Engineering Division, Chinese Society of Microbiology

1999锟斤拷 Expert Board for New Drug Evaluation, National Drug Administration, SDA

2002锟斤拷 Expert Board for Biochemistry and Molecular Biology Division, National Natural Science Foundation of China

2000锟斤拷 Academic Committee Member, Key Laboratory of Molecular Enzyme Engineering, Jilin University

2000锟斤拷 Academic Committee Member, Center for Human Disease Genomics, School of Medicine, Peking University

2000锟斤拷 Executive Editor, Chinese Journal of Biochemistry and Molecular Biology

2002锟斤拷 Editor, Acta Biochimica et Biophysica Sinica

E, Publications:

[1] Tang,J.G., Wang,C.C. & Tsou, C.L., (1988) Formation of native insulin

from scrambled molecule by protein disulfide isomerase., Biochemical Journal, 255, 451-455

[2] Tang,J.G. & Tsou,C L., (1990) The insulin A and B chains contain structural information for the formation of the native molecule. Studies with protein disulfide isomerase., Biochemical Journal, 268, 429-435

[3] 锟狡斤拷锟斤拷, (1991) 锟斤拷锟斤拷锟绞讹拷锟斤拷锟斤拷旃姑�----一锟街匡拷锟杰诧拷锟诫蛋锟斤拷锟斤拷锟斤拷锟斤拷铣傻锟矫�, 锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷展, 18, 173-176 (Protein disulfide isomerase锟斤拷an enzyme may be involved in protein biosynthesis, Progress in Biochemistry and Biophysics)

[4] 锟狡斤拷锟斤拷, (1991) 锟斤拷锟斤拷锟斤拷锟皆拷锟斤拷岵伙拷锟斤拷氲帮拷锟斤拷识锟斤拷锟斤拷锟届构酶锟斤拷锟斤拷锟斤拷, 锟斤拷锟斤化学锟斤拷志, 7, 638-640 (3T-thyroxine does not bind specifically to protein disulfide isomerase, Chinese Journal of Biochemistry)

[5] 锟狡斤拷锟斤拷, 薛英锟斤拷, 锟斤拷锟酵憋拷, 锟斤拷锟斤拷锟斤拷, (1993) 249锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷鹊锟斤拷锟皆拷诤系锟斤拷椎谋锟斤拷锟�, 锟斤拷锟斤工锟斤拷学锟斤拷, 9, 101-106 (Gene expression of a human proinsulin fusion protein with 249 amino acids, Chinese Journal of Biotechnology)

[6] 锟狡斤拷锟斤拷,(1993) 锟斤拷锟饺碉拷锟斤拷酶转锟侥的凤拷锟斤拷使锟斤拷锟饺碉拷锟斤拷原转锟斤拷锟斤拷锟饺碉拷锟斤拷, 锟斤拷锟斤工锟斤拷学锟斤拷, 9, 355-360 (Transpeptidation of human proinsulin to insulin by trypsin, Chinese Journal of Biotechnology)

[7] Tang,J.G. & Hu,M.H., (1993) Production of human proinsulin in E.coli in a non-fusion form., Biotechnology Letters, 15, 661-666

[8] Dai,Y. & Tang,J.G., (1994) Intra-A chain disulfide bond (A6-11) of insulin is essential for displaying its activity., Biochemistry and Molecular Biology International, 33, 1049-1053

[9] 锟狡斤拷锟斤拷, 锟脚猴拷锟斤拷, 锟斤怀锟斤拷, 锟斤拷锟斤拷, 锟斤拷锟斤拷锟斤拷, (1994) 锟饺碉拷锟斤拷B锟斤拷N锟剿碉拷锟斤拷锟轿硷拷锟斤拷锟斤拷锟斤拷学锟斤拷锟斤拷锟�, 锟斤拷锟斤化学锟斤拷志, 10, 665-669 (Insight into the importance of insulin B-chain N-terminal by modification, Chinese Journal of Biochemistry)

[10] 韦锟斤拷, 锟脚憋拷锟斤拷, 锟狡斤拷锟斤拷, 锟斤拷锟斤拷锟斤拷, (1994) 6锟斤拷锟斤拷锟斤拷锟斤拷小C锟斤拷锟斤拷锟饺碉拷锟斤拷原锟斤拷锟斤拷锟斤拷锟斤拷锟侥癸拷锟斤拷锟斤拷锟斤拷锟斤拷, 锟斤拷锟斤拷锟斤拷学学锟斤拷, 30, 722-727 (Construction and expression of mini-proinsulin with 6 amino acids as connecting peptide, Acta Scientiarum Naturalium Universitatis Pekinnensis)

[11] Wei,G., Hu,M.H. & Tang,J.G., (1995) Double-C-peptide human proinsulin. Biochemistry and Molecular Biology International, 35, 37-46

[12] 锟斤拷锟桔憋拷, 谢锟斤拷, 锟斤拷锟斤拷同, 锟狡斤拷锟斤拷, 锟斤拷锟斤拷锟斤拷, (1995) 锟斤拷锟饺碉拷锟斤拷原锟斤拷锟斤拷锟斤拷(B-Arg-Arg-A)锟斤拷锟斤拷墓锟斤拷锟斤拷捅锟斤拷锟�, 锟斤拷锟斤拷锟斤拷学学锟斤拷, 31, 84-91 (Construction and expression of B-Arg-Arg-A proinsulin, Acta Scientiarum Naturalium Universitatis Pekinnensis)

[13] Sun,H.Y., Tang,J.G. & Hu,M.H., (1995) Preparation of Tyr-C-peptide from genetically altered human insulin precursor., Applied Biochemistry and Biotechnology, 55, 167-174

[14] Chen,J.Q., Zhang,H.T., Hu,M.H. & Tang,J.G., (1995) Production of human insulin in E. coli system with met-lys-human proinsulin as the expressed precursor., Applied Biochemistry and Biotechnology, 55, 5-15

[15] Wei,G. & Tang,J.G., (1995) Formation of inclusion bodies may be the key factor for the stability of the expressed product in E.coli, Biochemistry and Molecular Biology International, 37, 895-901

[16] 锟斤拷锟斤拷同, 锟狡斤拷锟斤拷, 锟斤拷锟斤拷锟斤拷, (1995) 锟斤拷锟斤拷锟斤拷锟饺碉拷锟斤拷突锟斤拷锟斤拷(GluA4Leu)锟侥凤拷锟诫纯锟斤拷锟斤拷锟斤拷锟斤拷锟叫撅拷, 锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷展, 22, 40-43 (Purification and activity studies of GluA4Leu insulin, Progress in Biochemistry and Biophysics)

[17] Dai,Y. & Tang,J.G., (1996) Characteristic, activity and conformational studies of [A6-Ser, A11-Ser]-insulin., Biochimica et Biophysica Acta, 1296, 63-68

[18] 锟斤拷锟斤拷锟斤拷锟狡斤拷锟斤拷锟斤拷锟斤拷庭锟斤拷锟斤拷锟斤拷锟斤拷锟借，(1996) 锟饺碉拷锟斤拷酶锟斤拷锟斤拷锟斤拷锟揭拷缘锟斤拷芯锟斤拷锟斤拷锟斤拷锘э拷锟街撅拷锟�12, 583-587 (Role of disulfide bond in trypsin, Chinese Journal of Biochemistry)

[19] 锟脚讹拷锟结，锟狡斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟借，(1996) 锟饺碉拷锟斤拷酶锟斤拷锟皆的讹拷锟斤拷锟解定锟斤拷锟斤拷锟斤拷锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷展锟斤拷23, 551-553 (A novel method for the activity determination of trypsin, Progress in Biochemistry and Biophysics)

[20] Li, X.F., Tang, J.G. & Zhang, L.X., Autolytic site mutant R105C of rat trypsin. (1997) Biochemistry and Molecular Biology International, 42, 991-996

[21] 锟斤拷锟斤拷锟斤拷锟斤拷锟狡斤拷锟斤拷锟斤拷(1997) 锟剿硷拷状锟斤拷锟劫硷拷锟截的伙拷锟斤拷锟铰★拷锟斤拷诖蟪Ω司锟斤拷械谋锟斤拷铮拷锟斤拷锕わ拷锟窖э拷锟斤拷锟�13锟斤拷264-268 Human parathyroid hormone gene cloning and expression in E. coli, Chinese Journal of Biotechnology)

[22] Du, X. & Tang, J.G., (1998) Effects of deleting A19 tyrosine from insulin. Biochemistry Molecular Biology International, 44, 507-513

[23] 锟狡斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷斐わ拷锟斤拷锟斤拷锟斤拷锟斤拷疲锟斤拷锟绞ワ拷锟斤拷锟�(1998) 锟斤拷锟斤拷锟绞癸拷锟教碉拷锟叫撅拷锟斤拷锟斤拷锟斤拷锟斤拷学学锟斤拷锟斤拷34锟斤拷342-349 (Insight into protein engineering, Acta Scientiarum Naturalium Universitatis Pekinnensis)

[24] Du, X. & Tang, J.G., (1998) Hydroxyl group of insulin A19 tyrosine is essential for receptor binding: studies on (A19-Phe) insulin. Biochemistry Molecular Biology International, 45, 255-260

[25] Nie, X. & Tang, J.G., (1998) RGD-containing trypsin with both platelet aggregation inhibitory activity and proteolytic activity. Biochemistry Molecular Biology International, 45, 1149-1154

[26] 锟斤拷锟斤拷锟斤拷锟斤拷锟狡斤拷锟斤拷锟斤拷(1998) 锟剿硷拷状锟斤拷锟劫硷拷锟截碉拷锟叫撅拷锟斤拷锟斤拷锟斤技锟斤拷通讯, 9, 145-148 (Progress on the study of human parathyroid hormone, Biotechnology Communications)

[27] 锟斤拷锟斤拷瘢锟斤拷锟揭伙拷锟斤拷锟斤拷锟斤拷瑁拷芎欤拷锟斤拷胤锟斤拷锟�锟狡斤拷锟斤拷锟斤拷(1998) 锟叫癸拷锟剿凤拷锟斤拷锟斤拷锟斤拷cDNA锟侥匡拷隆锟斤拷锟节大肠杆撅拷锟叫的筹拷锟斤拷锟斤拷锟斤，锟叫伙拷锟斤拷锟斤拷锟斤拷志锟斤拷36, 40-43 (Leptin gene cloning from Chinese individual and its expression in E. coli, Chinese Journal of Pediatr.)

[28] Li, X.F., Nie, X, & Tang, J.G., (1998) Anti-autolysis of trypsin by modification of autolytic site Arg117. Biochem. Biophys. Res. Commun., 250, 235-239

[29] Howard, M.J., Fuller, C., Broadhurst, R.W., Perham, R.N., Tang, J.G., Quinn, J., Diamond, A.G., & Yeaman, S.J. (1998) Three-dimensional structure of the major autoantigen in primary biliary cirrhosis. Gastroenterology, 115, 139-146

[30] 锟斤拷锟斤拷锟斤拷锟狡斤拷锟斤拷锟斤拷(1998) 锟饺碉拷锟斤拷锟斤拷锟斤拷榈硷拷锟斤拷藕糯锟斤拷荩锟斤拷锟斤拷锘э拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟秸癸拷锟�25(5)锟斤拷43-47 (Signal transduction via insulin receptor, Progress in Biochemistry and Biophysics)

[31] Yang, Z.H., Du, X., & Tang, J.G., (1999) Separation and characterization of trypsin and carboxypeptidase B digested products of Met-Lys-human proinsulin. Applied Biochemistry and Biotechnology, 76, 107-114

[32] Yuan, Y., Wang, Z.H., & Tang, J.G., (1999) Intra-A chain disulfide bond forms first during insulin precursor folding. Biochemical Journal, 343, 139-144

[33] 锟斤拷锟斤拷蕙锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟狡斤拷锟斤拷锟斤拷锟斤拷1999锟斤拷Met-Lys-双C锟斤拷锟斤拷锟饺碉拷锟斤拷原锟斤拷锟斤拷墓锟斤拷锟斤拷锟斤拷锛帮拷锟斤拷氪匡拷锟斤拷锟斤拷泄锟斤拷锟斤拷锘э拷锟斤拷锟斤拷锟斤拷锟斤拷学锟斤拷锟斤拷15锟斤拷558锟斤拷562 (Construction and purification of Met-Lys-double-C-peptide proinsulin, Chinese Journal of Biochemistry and Molecular Biology)

[34] Jing, J., & Tang, J.G., (2000) Platelet aggregation inhibitory activity of mutant proinsulin with C-peptide replaced by CRGDSC sequence. Biotechnology Letters, 22, 47-52

[35] 锟斤拷锟斤拷锟斤拷锟狡斤拷锟斤拷锟斤拷锟斤拷2000锟斤拷锟斤拷锟斤拷脂锟角碉拷泳锟斤拷锟斤拷锟叫伙拷锟斤拷DNA锟斤拷一锟街硷拷惴斤拷锟斤拷锟斤拷泄锟揭窖э拷糯锟窖э拷锟街撅拷锟�17锟斤拷51锟斤拷53 (A simple method for the recovery of DNA fragment from the gel, Chinese Journal of Medical Genetics)

[36] 锟斤拷锟斤拷锟街ｏ拷锟斤拷锟斤拷锟斤拷锟狡斤拷锟斤拷锟斤拷锟斤拷2000锟斤拷锟斤拷A6-Ala, A11-Ala锟斤拷-锟斤拷锟饺碉拷锟斤拷突锟斤拷锟斤拷墓锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟皆ｏ拷锟叫癸拷锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟斤拷锟窖э拷锟斤拷锟�16锟斤拷621锟斤拷615 (Studies of A6,A11Ala-insulin, Chinese Journal of Biochemistry and Molecular Biology)

[37] 锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷一锟斤拷锟斤拷锟斤拷锟截ｏ拷锟狡斤拷锟斤拷锟斤拷锟斤拷2000锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷诖蟪Ω司锟斤拷械拇锟斤拷模锟斤拷锟斤拷锟斤拷锟叫伙拷锟斤拷锟斤拷锟斤拷志, 38锟斤拷365锟斤拷368 (Expression and purification of human leptin from E. coli, Chinese Journal of Pediatr.)

[38] Yang, Z.H., Jing, J., & Tang, J.G., (2001) Inhibition of platelet aggregation of a mutant proinsulin molecule engineered by introduction of a native Arg-Gly-Asp sequence. Applied Biochemistry and Biotechnology, 90, 1-10

[39] Yin, D., & Tang, J.G., (2001) Gene therapy for streptozotocin-induced diabetic mice by electroporational transfer of naked human insulin precursor DNA into skeletal muscle in vivo. FEBS Letters, 495, 16-20

[40] Tang, J. G., & Koeffler, H.P., (2001) Structural and functional studies of CCAAT/Enhancer-binding protein epsilon. J Biol Chem.,276, 17739-46

[41] 锟斤拷锟斤拷锟斤拷锟斤拷锟狡斤拷锟斤拷锟斤拷锟斤拷2002锟斤拷锟斤拷锟斤拷锟节凤拷锟接帮拷锟铰ｏ拷Pro锟斤拷锟节碉拷锟斤拷锟斤拷锟桔碉拷锟叫碉拷锟斤拷锟矫ｏ拷锟斤拷锟斤拷锟斤拷学锟斤拷14锟斤拷35锟斤拷39 (Intra-molecular chaperon锟斤拷role of pro peptide in protein folding, Chinese Bulletin of Life Sciences)

[42] 锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟侥ｏ拷锟狡斤拷锟斤拷锟斤拷锟斤拷2002锟斤拷锟斤拷锟缴筹拷锟斤拷原锟斤拷锟斤拷锟斤拷锟节大肠杆撅拷锟叫的憋拷锟斤、锟斤拷锟诫纯锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷学锟斤拷锟斤拷34, 595-600 (Expression, purification and characterization of human prorelaxin-like-protein H2 in E. coli, Acta Biochimica et Biophysica Sinica)

[43] Chen, L.M., Yang, X.W., & Tang, J.G. (2002) Acidic residues on the N-terminus of proinsulin C-peptide are important for the folding of insulin precursor. J Biochem., 131, 855-59

[44] Yang, J.C., Liu, J., Yang, X.W., & Tang, J.G. (2002)Gene therapy for diabetic rats by electroporational transfer of naked plasmid with human preproinsulin gene into skeletal muscle. Biotechnology Letters, 24, 851-855

[45] 锟斤拷锟斤拷锟侥ｏ拷锟斤拷锟斤拷锟狡斤拷锟斤拷锟斤拷锟斤拷2002锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷RGD226锟斤拷锟津构斤拷锟斤拷锟斤拷锟斤、锟斤拷锟斤纯锟斤拷锟斤拷锟斤拷锟皆凤拷锟斤拷锟斤拷锟叫癸拷锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟斤拷锟窖э拷锟�, 18, 583-587 (Gene construction, expression, purification and activity analysis of anti-platelet aggregation peptide RGD226, Chinese Journal of Biochemistry and Molecular Biology)

[46] Liu, Y., & Tang, J.G. (2003) Influence of A7-B7 disulfide bond deletion on the refolding and structure of proinsulin. Acta Biochimica et Biophysica Sinica, 35(2), 122-126

[47] Liu, J., Yang, X.W., Chen, W.Q., & Tang, J.G. (2003) Effect of disulfide bond formation on the conformation and anticoagulant activity of an Arg-Gly-Asp motif displayed on a mutant insulin protein framework. Letters in Peptide Science, in press

[48] Tang, J.G., Wang, Z.H., Tregear, G.W., & Wade, J.D. (2003) Human gene 2 relaxin chain combination and folding. Biochemistry, 42(9), 2731-2739

[49] Wang, Z.H., Liu, Y., Yang, X.W., & Tang, J.G. (2003) Probing the role of disulfide bonds in insulin folding and activity. In preparation

[50] Wang, X.D., Liu, J., Yang, J.C., Chen, W.Q., & Tang J.G. (2003) Mice body weight gain is prevented after naked human leptin cDNA transfer into skeletal muscle by electroporation. In preparation

[51] Yang, J.C. & Tang J.G. (2003) Aromatic side chain of Tyr61is important for structure and function of leptin. In preparation

F, Patents:

[1] 锟狡斤拷锟斤拷, 锟斤拷锟斤拷锟斤拷, 锟饺碉拷锟斤拷前锟斤拷锟斤拷锟斤拷诖蟪Ω司锟斤拷械锟街憋拷颖锟斤拷锛帮拷锟接癸拷锟斤拷锟斤拷, 锟叫癸拷专锟斤拷, 专锟斤拷锟斤拷: 93103997.5 (Direct expression of insulin precursor gene in E. coli and simple procedure for preparation of human insulin)

[2] 锟狡斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷志锟疥，锟斤拷锟睫伙拷锟斤拷锟饺碉拷锟斤拷为锟角架的猴拷RGD锟斤拷锟叫的匡拷栓锟斤拷药锟斤拷锟叫癸拷专锟斤拷锟斤拷锟斤拷锟斤拷牛锟�99109419.0 (Anticoagulant RGD motif displayed on an inactive insulin protein framework, patent in pending)

[3] 锟狡斤拷锟斤拷锟斤拷锟斤拷锟杰ｏ拷锟斤拷锟斤拷锟斤拷一锟斤拷锟斤拷锟斤拷直锟接硷拷锟斤拷注锟斤拷锟斤拷锟斤拷锟斤拷锟津病碉拷锟斤拷锟饺碉拷锟斤拷前锟斤拷锟斤拷锟酵猴拷锟叫该伙拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷泄锟阶拷锟斤拷锟斤拷锟斤拷锟脚ｏ拷00129717.1 (A plasmid with insulin precursor gene for treatment of diabetes by electroporational transfer into skeletal muscle, patent in pending)

G, Book锟斤拷

锟斤拷锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟芥，锟斤拷2002锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷圣锟斤拷锟届长锟斤拷锟斤拷锟洁，锟竭等斤拷锟斤拷锟斤拷锟斤拷锟界，锟斤拷锟斤拷锟斤拷锟�4锟斤拷写锟斤拷锟斤拷(A text book on Biochemistry used national wide, third edition, I contributed 4 chapters. Published by Higher Education Press)