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一锟斤拷锟斤拷锟斤拷锟斤拷锟�

1974.12	1978.4	锟叫癸拷锟斤拷锟斤拷锟脚撅拷锟斤拷山要锟斤拷锟斤拷锟斤拷锟斤拷锟桔ｏ拷
1978.4	1978.8	锟姐江省锟斤拷锟斤拷锟斤拷山锟斤拷锟斤拷农锟斤拷
1978.9	1982.7	锟姐江农业锟斤拷学农学系锟斤拷学士锟斤拷
1982.8	1985.12	锟姐江农业锟斤拷学锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟教ｏ拷
1986.1	1989.12	锟斤拷锟斤拷锟斤拷锟轿讹拷锟斤拷学植锟斤拷系锟斤拷锟斤拷士锟斤拷锟斤拷锟斤拷士锟斤拷
1989.12	1991.6	锟斤拷锟斤拷锟斤拷盛锟劫达拷学锟斤拷锟斤拷系锟斤拷锟斤拷士锟斤拷
1991.6	1992.6	锟斤拷锟斤拷锟斤拷学锟斤拷锟斤拷系锟斤拷锟斤拷士锟斤拷
1992.7	1994.6	锟斤拷锟斤拷锟斤拷学锟斤拷锟斤拷锟斤拷学学院锟斤拷锟斤拷锟节ｏ拷锟斤拷锟斤拷锟绞癸拷锟教硷拷植锟斤拷锟斤拷蚬こ坦锟斤拷锟斤拷氐锟绞碉拷锟斤拷腋锟斤拷锟斤拷危锟窖э拷锟轿憋拷锟轿憋拷锟�
1994.7	锟斤拷锟斤拷	锟斤拷锟斤拷锟斤拷学锟斤拷锟斤拷锟斤拷学学院锟斤拷锟节ｏ拷锟斤拷士锟斤拷锟斤拷师锟斤拷学锟斤拷委员锟斤拷委员锟斤拷
1995.9	1997.3	锟斤拷锟斤拷锟斤拷锟捷诧拷锟斤拷锟斤拷锟斤拷学植锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟侥凤拷锟绞斤拷锟节ｏ拷
1999.7	锟斤拷锟斤拷	锟斤拷锟斤拷锟斤拷学锟斤拷学锟斤拷学锟斤拷委员锟斤拷委员锟斤拷
2000.7	锟斤拷锟斤拷	锟斤拷锟斤拷锟斤拷锟斤拷学锟斤拷锟斤拷委员锟斤拷锟斤拷锟斤拷锟斤拷学学锟斤拷委员
2001.7	锟斤拷锟斤拷	锟狡硷拷锟斤拷863锟竭硷拷锟斤拷锟狡伙拷锟斤拷锟斤拷锟斤拷锟街达拷农业锟斤拷锟斤拷专锟斤拷委员锟斤拷委员
2001.9	锟斤拷锟斤拷	锟斤拷锟斤拷锟斤拷聘锟斤拷锟斤拷
2002.5	锟斤拷锟斤拷	农业锟斤拷锟斤拷锟斤拷转锟斤拷锟斤拷锟斤拷锟斤安全委员锟斤拷委员
2002.9	锟斤拷锟斤拷	锟斤拷锟斤拷锟绞癸拷锟教硷拷植锟斤拷锟斤拷蚬こ坦锟斤拷锟斤拷氐锟绞碉拷锟斤拷锟斤拷锟斤拷锟�

Major Research Areas:

My laboratory is interested in two major research areas: large-scale cloning and analyses of genes involved in early cotton fiber development, and tuberculosis DNA vaccine development. Using cDNA prepared from 10dpa (days post anthesis) wild-type cotton fiber as tester and cDNA from a fuzzless-lintless (fl) mutant as driver, we applied the RDA method to recover 280 independent cDNA fragments related directly to early cotton fiber development. cDNA microarrays showed that 172 genes were significantly up-regulated in elongating cotton fibers as confirmed by in situ hybridization in representative cases. Twenty-nine cDNAs, including a putative vacuolar (H⁺)-ATPase catalytic subunit, a kinesin-like calmodulin binding protein, several arabinogalactan proteins and key enzymes involved in long chain fatty acid biosynthesis, accumulated to greater than 50-fold in 10 dpa fiber cells when compared to that in 0 dpa ovules. Various upstream pathways, such as auxin signal transduction, the MAP kinase pathway, and profilin- and expansin-induced cell wall loosening, were also activated during the fast fiber elongation period.

We have cloned five genes encoding different secreted antigens Ag85B, MPT-83, MPT-64, MPT-63 and ESAT-6 from Mycobacterium Tuberculosis. We are currently developing either combined or polyvalent DNA vaccines with different combinations. Experimental results obtained both from mice and from cows showed that immunization of combined DNA vaccines expressing 3 or 4 secreted antigens might produce significant protection against microbial invasion. Our data indicated that antigen-specific IFN-锟斤拷levels, rather than antibody levels, might be a better criteria for protective efficacy assessment.

Besides the above two major areas, the lab is also involved in cloning and expressional analyses of 8 large gene families (AP2-EREBP锟斤拷bHLH锟斤拷C3H-TYPE2(Zn)锟斤拷E2F-DP锟斤拷HMG-BOX锟斤拷NIN-like锟斤拷PCG锟斤拷TCP) encoding Arabidopsis transcription factors. We have so far obtained about 250 full-length cDNAs and are currently working on yeast expression as well as gel mobility shift assays to study their DNA binding activities. We have done some work on elucidating molecular mechanisms of plant apical senescence using G2 pea as a model system. We showed that transgenic Arabidopsis plants carrying a putative chloroplast membrane-localized calcium channel protein (the PPF1 gene) in sense orientation flowered much later compared to control plants whereas antisense expression of this gene rendered the recipients a very rapid life cycle. The strength of this late-flowering phenotype correlated roughly with the level of PPF1 transcripts and with the calcium storage capacity in the chloroplasts of different transgenic lines. We suggest that PPF1 is a novel calcium ion carrier that regulates flowering time of higher plants by changing the Ca²⁺ storage capacity within chloroplasts.

锟斤拷锟斤拷锟斤拷要锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫筹拷2000锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟侥ｏ拷

*Sheng-Jian Ji, Ying-Chun Lu, Jian-Xun Feng, Gang Wei, Jun Li, Yong-Hui Shi, Qiang Fu, Di Liu, Jing-Chu Luo and Yu-Xian Zhu. (2003) Isolation and Analyses of Genes Preferentially Expressed During Early Cotton Fiber Development by Subtractive PCR and cDNA Array. Nucleic Acids Res. 31: in press.

*锟教宏，锟斤拷霞锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟�庄锟斤拷裕锟斤拷锟斤拷锟斤拷汀锟�(2003) 锟斤拷朔锟街︼拷司锟斤拷募酆锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟皆拷院捅锟斤拷锟叫э拷锟斤拷芯锟斤拷锟�锟叫癸拷锟斤拷学锟斤拷印刷锟叫★拷

锟斤拷锟斤拷坦锟斤拷锟斤拷锟酵白匡拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟酵★拷(2003) 锟斤拷锟斤拷蝎锟斤拷锟芥毒锟斤拷AaHIT1锟斤拷锟斤拷锟皆拷吮锟斤拷锟斤拷转锟斤拷锟斤拷锟叫撅拷锟斤拷锟叫癸拷锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟斤拷锟窖э拷锟斤拷锟�19锟斤拷182-185锟斤拷

锟斤拷锟斤拷锟斤拷锟教宏，锟斤拷锟斤拷霞锟斤拷锟斤拷锟狡ｏ拷锟斤拷锟斤拷锟酵★拷(2003) MPT63锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷票锟斤拷锟斤拷锟斤拷锟斤拷锟皆拷浴锟�锟叫癸拷锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟斤拷锟窖э拷锟斤拷锟�19锟斤拷205-209锟斤拷

*Dayong Wang, Yunjian Xu, Qing Li, Xuemei Hao, Yanhui Su, Fangzhen Sun, Yuxian Zhu. (2003) Transgenic expression of a putative calcium transporter affects the time of arabidopsis flowering. The Plant Journal锟斤拷33: 285-292

*锟斤拷锟狡斤拷锟斤拷锟斤拷雪锟缴ｏ拷锟斤拷B锟斤拷锟斤拷锟界，Peter J. Davies锟斤拷锟斤拷锟斤拷锟酵★拷锟斤拷2003锟斤拷锟姐豆锟斤拷锟斤拷锟斤拷锟结还原锟届构酶锟斤拷锟斤拷峁癸拷锟斤拷锟斤拷谆锟斤拷苑锟斤拷锟斤拷锟斤拷锟斤拷锟截伙拷锟斤拷锟叫撅拷锟斤拷锟斤拷锟叫癸拷锟斤拷学锟斤拷锟斤拷33: 19-26

*锟斤拷锟斤拷锟斤拷锟教宏，锟斤拷锟斤拷霞锟斤拷锟斤拷霞锟斤拷锟斤拷锟狡ｏ拷锟斤拷锟斤拷锟酵★拷(2003) 锟斤拷朔锟街︼拷司锟斤拷锟斤拷DNA锟斤拷锟斤拷锟斤拷锟斤拷锟叫э拷锟斤拷芯锟斤拷锟斤拷锟斤拷锟斤拷锘э拷锟斤拷锟斤拷锟斤拷锟斤拷锟窖э拷锟斤拷锟斤拷锟�35: 71-76锟斤拷

锟斤拷锟斤拷坦锟斤拷锟斤拷锟酵白匡拷锟斤拷锟斤拷锟斤拷锟铰ｏ拷锟斤拷锟斤拷锟酵★拷(2003) GDA2锟斤拷锟斤拷目锟铰★拷锟皆拷吮锟斤拷锟斤拷锟斤拷诤系锟斤拷椎拇锟斤拷锟斤拷锟�锟斤拷农业锟斤拷锟斤技锟斤拷学锟斤拷锟斤拷锟斤拷11: 6-10锟斤拷

*Yun-Jian Xu, Da-Yong Wang, Yu-Xian Zhu (2002) Expression of the thylakoid membrane localized PPF1 in transgenic Arabidopsis affects chloroplast development. Acta Botanica Sinica, 41: 1314-1320锟斤拷

锟斤拷锟狡斤拷锟斤拷锟斤拷锟斤拷坦锟斤拷锟斤拷锟酵白匡拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟酵★拷锟斤拷2002锟斤拷PPF1锟斤拷锟斤拷C锟斤拷片锟斤拷锟节大肠杆撅拷锟叫碉拷锟节合憋拷锟斤拷痛锟斤拷锟斤拷约锟斤拷锟斤拷锟斤拷票锟斤拷锟斤拷锟斤拷泄锟斤拷锟斤拷锘э拷锟斤拷锟斤拷锟斤拷锟斤拷学锟斤拷锟斤拷锟斤拷18: 594-599锟斤拷

锟教宏，锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟酵★拷锟斤拷2002锟斤拷锟斤拷朔锟街︼拷司锟�MPT-64锟斤拷锟节碉拷锟斤拷锟斤拷原锟剿憋拷锟斤拷锟斤拷锟斤拷锟叫的匡拷隆锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷泄锟斤拷锟斤拷薰锟斤拷锟斤拷锟斤拷锟街撅拷锟斤拷锟�18(4):1-4

锟教宏，锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟酵★拷锟斤拷2002锟斤拷锟斤拷朔锟街︼拷司锟斤拷锟斤拷址锟斤拷诘锟斤拷锟斤拷锟皆拷吮锟斤拷锟斤拷锟斤拷锟斤拷械目锟铰★拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫癸拷预锟斤拷锟斤拷医学锟斤拷锟斤拷锟斤拷5锟斤拷24锟斤拷325-330

*锟教宏，锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟阶拷锟皆ｏ拷锟斤拷锟斤拷锟酵ｏ拷锟斤拷2002锟斤拷锟斤拷朔锟街︼拷司锟斤拷锟桔猴拷锟斤拷锟斤拷锟斤拷墓锟斤拷锟斤拷锟斤拷锟斤拷锟皆拷浴锟斤拷锟斤拷锟窖拷锟斤拷锟斤拷锟�47: 966-971. Cai Hong, Pan Yi, Li Guoli, Zhuang Yuhui, Zhu Yuxian. (2002) Construction of multivalent DNA vaccines for Mycobacterium tuberculosis and its immunogenicity. Chinese Science Bulletin, 47(19): 1589-1593

*Sheng-Jian Ji, Feng Liu, Er-Qiu Li, Yu-Xian Zhu. (2002) Recombinant scorpion insectotoxin AaIT kills specifically insect cells but not human cells. Cell research, 12(2): 143-150.

*Shengjian Ji, Yingchun Lu, Jun Li, Gang Wei, Xiujuan Liang, Yuxian Zhu. (2002) A b-tubulin-like cDNA expressed specically in elongating cotton fibers induces longitudinal growth of fission yeast. Biochemical and Biophysical Research Communications. 296 (5): 1245-1250.

*Lu Yingchun, Wei Gang, Zhu Yuxian (2002) Clong whole cellulose-synthesizing operon (ayacs Operon) from Acetobacter xylinum and transforming it into cultivated cotton plants. Acta Botanica Sinica. 44: 441-445

*Wang Dayong, Hu Shuang, Li Qing, Cui Keming and Zhu Yuxian (2002) Photoperiod control of the apical bud and leaf senescence in pumpkin strain 185. Acta Botanica Sinica. 44: 55-62

*锟斤拷锟斤拷裕锟斤拷锟斤拷欤�Vincent Vachon锟斤拷锟脚杰ｏ拷锟睫撅拷锟斤拷锟斤拷Raynald Laprade锟斤拷锟斤拷锟斤拷锟酵ｏ拷2002锟斤拷锟斤拷锟狡斤拷芽锟竭杆撅拷Cry1Aa锟斤拷锟阶э拷4锟斤拷锟斤拷锟轿成匡拷膜锟斤拷锟斤拷通锟斤拷锟斤拷锟斤拷锟芥毒锟斤拷锟斤拷锟斤拷锟斤拷模锟斤拷预锟解。锟斤拷锟叫癸拷锟斤拷学锟斤拷锟斤拷32: 146-152锟斤拷SuYanhui, QuHong, VACHON Vincent, Lu Jingchu, ZHANG Jie, LAPRADE Raynald and ZhuYuxian (2002) The toxicity test and hypothetical model of Bacillus thuringiensis Cry1Aa helix4. Science In China. 45: 561-567

锟教宏，锟斤拷锟斤拷锟酵ｏ拷2001锟斤拷锟斤拷烁司锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫撅拷锟斤拷展锟斤拷锟叫癸拷预锟斤拷锟斤拷医学锟斤拷展锟斤拷4锟斤拷5-9锟斤拷

*锟届华锟缴ｏ拷锟斤拷锟狡斤拷锟斤拷锟斤拷雪锟缴ｏ拷锟斤拷廷锟铰ｏ拷锟斤拷锟斤拷裕锟斤拷锟斤拷锟斤拷停锟�2001锟斤拷锟姐豆锟斤拷锟斤拷锟斤拷锟结还原锟届构酶锟斤拷锟斤拷锟�cDNA锟斤拷隆锟斤拷锟斤拷锟叫凤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷芯锟斤拷锟斤拷锟窖拷锟斤拷锟�46锟斤拷1022-1026锟斤拷Xu Huasong, Xu Yunjian, Gu Xuesong, Hu Tingzhang, Su Yanhui and Zhu Yuxian (2001) Cloning and analysis of a cDNA encoding acetohydroxy acid isomeroreductase from G2 pea. Chinese Science Bulletin, 46: 1808-1812. IF 0.511

*Junli Zhou, Ligeng Ma, Suqiao Zhang, Yuxian Zhu, Daye Sun. (2001) Extracellular calmodulin stimulates light-independent RbcS-GUS expression in suspension-cultured cells of transgenic tobacco. Plant Cell Physiol, 42: 1049-1055. IF 2.30

*Guoying Jiang, Yunjian Xu, Xinsheng Zhu, Yanhui Su and Yuxian Zhu (2001) Prokaryotically expressed Buthus martensii Karsch insect depressant toxin has insecticidal effects. Toxicon. 39: 469-476. IF 1.366

锟教宏，锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷霞锟斤拷锟斤拷锟结，锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷纾拷锟斤拷锟斤拷停锟�2001锟斤拷锟斤拷锟斤拷PCR锟斤拷锟斤拷锟斤拷锟�537锟斤拷锟铰斤拷锟斤拷瞬锟斤拷锟教碉拷瓯撅拷械慕锟剿凤拷枝锟剿撅拷锟斤拷锟叫癸拷锟斤拷锟筋共锟斤拷锟斤拷锟斤拷志锟斤拷17: 65-67

锟教宏，锟斤拷锟斤拷霞锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷纾拷锟斤拷锟斤拷停锟�2001锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷酶锟斤拷片锟轿筹拷锟饺讹拷锟斤拷锟皆ｏ拷RFLP锟斤拷锟斤拷PCR指印锟斤拷锟斤拷锟斤拷牛锟斤拷朔锟街э拷司锟斤拷锟斤拷锟斤拷芯锟斤拷锟斤拷泄锟皆わ拷锟斤拷锟揭窖э拷锟斤拷锟�23锟斤拷175-179

*Huang Jianqiu, Wei Zhiming, An Hailong and Zhu Yuxian (2001) Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer. Cell Research, 11: 149-155. IF 2.00

*Yuxian Zhu, James M. Tepperman, Crag Fairchild and Peter H. Quail (2000) Phytochrome B binds with greater apparent affinity than phychrome A to the basic helix-loop-helix factor PIF3 in a reaction requiring the PAS domin of PIF3. Proc. Natl. Acad. Sci. 97:13419-13424

*锟斤拷学锟铰ｏ拷锟斤拷锟斤拷锟酵ｏ拷锟斤拷锟斤拷蕃锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫ｏ拷2000锟斤拷锟斤拷锟斤拷维锟斤拷锟�cDNA锟侥匡拷隆锟斤拷锟斤拷突锟斤拷锟斤拷锟叫的结构锟斤拷锟届。锟斤拷学通锟斤拷锟斤拷45: 1852-1857锟斤拷Xuede Wang, Yuxian Zhu, Daofan Ji, Shuli Jiang and Yuyou Li (2000) Cloning of cotton fiber-related cDNA and its structure variants in four cotton fiber mutants. Chinese Science Bulletin, 45: 1852-1857

锟斤拷锟斤拷裕锟斤拷锟斤拷平锟斤拷锟斤拷锟窖╋拷桑锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟� (2000) 锟斤拷锟斤拷锟斤拷PCR锟斤拷锟斤拷隆全锟斤拷cDNA锟斤拷锟斤拷锟斤化学锟斤拷锟斤拷锟斤拷锟斤拷锟窖аэ拷锟斤拷锟�16: 751-754

*锟斤拷廷锟铰ｏ拷锟斤拷锟狡斤拷锟斤拷锟斤拷锟斤拷英锟斤拷锟斤拷锟斤拷锟酵ｏ拷2000锟斤拷转BmKIT4锟斤拷锟斤拷锟教草的匡拷锟斤拷锟皆★拷植锟斤拷学锟斤拷锟斤拷42锟斤拷732锟斤拷735锟斤拷Tingzhang Hu, Yunjian Xu, Guoying Jiang and Yuxian Zhu (2000)锟斤拷Studies on insect resistance of transgenic tobacco plants carrying the BMK IT4 gene. Acta Botanica Sinica锟斤拷42锟斤拷732锟斤拷735