Reverse Oxygen Effect on γ-Irradiation
Induced
Single Strand Breaks of Plasmid DNA
SHAO Chun-Lin1 , SAITO Masahiro2 and
YU Zeng-Liang1
( 1 Institute of Plasma Physics, Chinese Academy of Sciences, P
O Box 1126, Hefei 230031, China；
2 Research Reactor Institute, Kyoto University, Osaka
590-0494, Japan )
Abstract Oxygen effect on γ-irradiation
induced single strand breaks (SSB) of plasmid DNA was measured by the method of
gel scanning. It was found that, in the presence of mannitol, the yield of SSB
in N2-saturated DNA solution was larger than that in air-saturated
solution so that the oxygen enhancement ratio (OER) of SSB became smaller than
1, which indicates the appearance of reverse oxygen effect of DNA SSB. At the
same time, G(SSB) and its OER decreased with increasing concentration of
mannitol.
Key Words γ-irradiation; DNA strand
breaks; reverse oxygen effect
Corresponding author:
Tel, 86-551-5591382; Fax, 86-551-5591310; e-mail, [email protected]