Electron Leak Causes Proton Leak in
Skeletal Muscle
Mitochondria in Exercise-induced Fatigue
SHI Qing-De, ZHANG Yong., CHEN Jia-Qi and LIU
Shu-Sen
( Tianjin Research Institute of Sports Medicine, Tianjin 300381, China；
..State Key Laboratory of Biomembrane & Membrane
Biotechnology, Institute of Zoology,
the Chinese Academy of Sciences, Beijing 100080, China )
Abstarct Using an animal model
of exercise-induced fatigue by incremental exercise to exhaustion on a
motor-driven treadmill, the changes of the mitochondrial proton leak and
electron leak in mitochondria of skeletal muscle from rats immediately after
exhaustive exercise were observed. The results showed that, in exercise-induced
fatigue conditions, superoxide anions of rat skeletal muscle mitochondria
increased；State 4 respiration rate significantly
accelerated and respiration control ratio clearly decreased. Results show that,
in the condition of exercise-induced fatigue, the increased mitochondrial
proton leak and electron leak may be important factors that cause the decrease
in coupling of oxidative phosphorylation. Our results provide evidence
supporting a hypothesis that electron leak causes proton leak in mitochondria.
Key Words Mitochondria; oxidative
phosphorylation; exercise-induced fatigue; electron leak; proton leak
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