Cloning of Glial Cell Line-derived
Neurotrophic Factor
Gene and Its Expression in Eukaryotic Cells
JIANG Qiong, LIU Jian, GE Kai, LIU Wei-Cheng, SUN Lan-Ying,
LIU Xin-Yuan and ZHENG Zhong-Cheng
( Shanghai Institute of Biochemistry, the Chinese Academy of Science, shanghai
200031, China )
Abstract Glial cell line-derived
neurotrophic factor gene (gdnf) was obtained by RT-PCR method. Its
pro-duct in COS7 cells showed neurotrophic and protective effect on the
dopaminergic neurons. Then gdnf gene was cloned into retrovirus vector pLNCX, and
packaged with PA317. Infectious particles thus obtained were used to infect rat
myoblast cell line L-6TG. A cell clone L-6TG/gdnf was obtained after
G418 selection. It will be applied to the ex vivo gene therapy study
of Parkinson’s disease.
Key Words Glial cell line-derived
neurotrophic factor; Parkinson’s disease; gene therapy
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