Localization of PS9 in Rice Ragged Stunt
Oryzavirus and Its Role in Virus Transmission by Brown Planthopper
LU Xiong-Bin, PENG Bao-Zhen, ZHOU Guo-Ying, and GONG Zu-Xun
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai
200031, China )
JIN Deng-Di,CHEN Sheng-Xiang
( Zhejiang Academy of Agricultural Science, Hangzhou 310021, China
)
Abstarct A cDNA of rice ragged stunt
oryzavirus (RRSV), coding for its protein PS9, was prepared and expressed in E.
coli as a fusion protein, then purified and cleaved by factor-Xa to a 38 kD
polypeptide. Using the IgG of the antiserum raised against the expressed fusion
protein, the gold immuno-labelling experiments provided a direct evidence that
the 38kD polypeptide is one of the major proteins forming the virus spikes.
Virus transmission experiments in brown planthopper fed with PS9 showed the
inhibition of transmission of virus by the PS9 protein, suggesting that the
spike proteins of the virus may be essential for the virus infection.
Key words Rice ragged stunt oryzavirus; segment
9; brown planthopper Nilaparvate lugens; spike protein; localization
and function
Corresponding author:
Tel, 86-21-64374430-220; Fax, 86-21-64746510; e-mail, [email protected]