Sequencing of a 3.3 kb Fragment from
LsNPV Genome and Comparison of Its Genome Structure with Other Two NPVs
LIU De-Li, JIN Tian-Quan, QI Yi-Peng and LI Li
( Institute
of Virology, Wuhan University, Wuhan 430072, China )
Abstract A fragment (3 336 bp) of LsNPV
genome has been sequenced. It contains three complete ORFs (ORF2,
ORF3, ORF4) and two incomplete ORFs (ORF1, ORF5).Comparing
with AcNPV genome, it was found that ORF1 coding product was
homologous with P94 protein; ORF3, ORF4, ORF5 were
homologous with ORF60, ORF59 and ORF57 of AcNPV, respectively.
Their transcriptional orientation are identical. It is suggested that the ORF2
is a new gene of LsNPV genome. The results showed that the genomic structure of
LsNPV is quite different with that of other baculovirus compared. This may be
helpful for investigation of the LsNPV molecular biology.
Key Words DNA sequencing; homologous
analysis; genomic structure; LsNPV
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