Yeast Two-hybrid System and Its
Application on Proteomics
YANG Qi-Heng and LI Lin*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract Proteomics is a new research
area of the post-genomic era that aims at the analysis and identification of
the entire proteins present in the cell, tissue or organism, and of the
functions and the linkages of these proteins. Protein-protein interactions are
characteristic of cellular activities and of course an important part of
proteomics. One of the effective techniques to detect protein-protein
interaction is yeast two-hybrid system. This system was successfully employed
to analyze the protein linkage map of the yeast mRNA splicing machinery, which
boded well for its application on human proteome research.
Key Words Yeast two-hybrid system； proteomics； protein-protein
interaction
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