Cloning of Human Glial Neurotrophic
Factor and Its
High Expression in Insect Cells
CHEN Zhe-Yu and LU Chang-Lin
( Department of Neurobiology, the Second Military Medical University, Shanghai
200433, China )
SUN Jian-Xin and WU Xiang-Fu*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai
200031, China )
Abstract The human glial cell derived
neurotrophic factor(GDNF)was expressed in Tn-5B1-4 insect cells using the Bac
to Bac baculovirus expression system, accounting for 30% of total cellular
soluble proteins. The expressed product was purified by affinity chromatography
and the activity assays showed that it could significantly promote the survival
of dopaminergic neurons. The study can be the basis for further
structural-functional analysis of GDNF.
Key Words Recombinant GDNF; baculovirus expression system(BES); dopaminergic neuron
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