Effects of ZNC(C)PR and
Its Analogs on CNDF mRNA Expression in Rat Brain
LI Wen-Xue, GU Ben-Xian and DU Yu-Cang*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract Reverse transcription(RT)-PCR
method was used to examine the cholinergic neuron derivative factor(CNDF)mRNA
level changes in rat brain after the administration of ZNC(C)PR or its analogs.
Glyceraldehyde-3-phophate dehydrogenase(GAPDH)mRNA was co-amplified as an
internal control. The CNDF mRNA expression was significantly increased and its
peak was reached at 18 h after the administration of ZNC(C)PR(hippocampus, 3.02
fold； cortex, 5.33 fold； compared to control, P
< 0.01). NLPR, agonist of ZNC(C)PR receptor, enhanced the CNDF mRNA
transcription to a higher level than ZNC(C)PR. ZDC(C)PR, antagonist of ZNC(C)PR
receptor, partially blocked the CNDF transcription when co-administered with
ZNC(C)PR. AVP increased the CNDF mRNA expression to lower level than ZNC(C)PR,
while oxytocin had no effect on the CNDF transcription. The results suggest
that the CNDF gene may be one of the targets of ZNC(C)PR, and its
enhancement effect on CNDF mRNA expression was mediated by the ZNC(C)PR
receptor.
Key Words ZNC(C)PR； CNDF； RT-PCR
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