Helicobacter pylori HspA
Heat-shock Protein Gene：
Cloning, Expression and Immunogenicity
LI Ming-Feng, LING Zhen, ZHANG Ying-Chun and MA Ai-Ying
( 455 Hospital of PLA, Shanghai 200052, China )
SUN Jian-Xin, SHI Hui-Juan and WU Xiang-Fu*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract Helicobacter pylori is the causative agent of
gastritis and peptic ulcer in human. The heat-shock protein A(HspA)may
stimulate the immunoresponse protecting human body against challenge of H.pylori.
The gene encoding the structural A subunit of H.pylori heat-shock
protein was amplified from H.pylori chromosomal DNA by PCR, and was
inserted in the prokaryotic expression vector pET-22b(+), and then was
transformed into the BL-21(DE3)E.coli strain to express the HspA
recombinant protein. HspA gene was measured to be 354 base pairs, and
the recombinant protein gene encoded polypeptides of 118 amino acid residues, corres-ponding
to calculated molecular weight of 15 kD. Western blot analysis of HspA
recombinant protein was confirmed that it could be specifically recognized by
the serum of H.pylori-infected patients, and could also be re-cognized
by the serum of immunized Balb/c mice with HspA itself. This result suggests
that HspA may be an effective protein vaccine for prevention and treatment of
the infection of H.pylori.
Key Words Helicobacter pylori； heat-shock protein A； cloning； expression； immunogenicity
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