cDNA Cloning of the Cytoplasmic Domain of
Growth Hormone Receptor from guinea Pig and Homology Comparison
ZHANG Xin-Na, SUN Xun and ZHU Shang-Quan*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract The evolutionary position of
guinea pig has not been unequivocally clarified, and it appears to show abnormalities
in GH response. The cDNA of GHR cytoplasmic domain from guinea pig was cloned
and sequenced. By homology comparison, it was found that the sequence we
obtained was different markedly from that of rat or mouse. The results provided
not only the molecular biology evidence for clarifying the evolutionary
position of guinea pig, but also a basis to determine the complete cDNA
sequence of GHR from guinea pig and to understand its cytoplasmic signal
transduction.
Key Words Guinea pig(Cavia porcellus)； growth hormone
receptor(GHR)；
cytoplasmic domain； sequencing； homology comparison
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