Inducible Expression of bcl-2
Antisense RNA Promotes Apoptosis of Human Gastric Cancer Cell Line
ZHANG Jie1*, PENG Wei-Dan2, HUI
Hong-Xiang2, JIN-Ming2, WANG Duo-Ning3,
WANG Cheng-Ji2 and QIAN Huan-Wen1
( 1 Institute of Radiation Medicine, Academy of Military Medical
Sciences, Beijing 100850, China;
2 Department of Biochemistry and Molecule Biology, Forth
Military Medical University, Xian 710033, China;
3 Department of Chemistry, Forth Military Medical University,
Xian 710033, China）
Abstract A lot of molecular biological
techniques was used to observe the effect of bcl-2 antisence RNA on
human gastric cancer cell line SGC7901 and to detect the inducibility and
promotion of MT-II promoter. The results showed that MT-II promoter could be
activated by the induction with 160 μmol/L Zn2+ and the expression
of Bcl-2 plays an important role in apoptosis of SGC7901 cells.
Key Words Antisense; bcl-2;
apoptosis; gene therapy
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