Expression of α and β Genes of Human
Hemoglobin in Escherichia coli
ZHANG Hao, WANG Quan-Li, FENG Qi, DU Zhi-Yan, LIU Xiao-Da,
LIAO Xing-Rong
and MAO Bing-Zhi1*
( Beijing Institute of Transfusion Medicine, Beijing 100850, China;
1Beijing Institute of Radiation Medicine, Beijing 100850, China
)
Abstract Total RNA was isolated from
human erythrocyte. cDNA fragments coding for alpha and beta genes of human
hemoglobins were obtained through RT-PCR and were ligated to plasmid pT7-Blue.
Then fragments of alpha and beta genes were cut off and
ligated with plasmid pBV220. Sequences identical compared with the published
ones were confirmed by DNA sequencing. Bacteria harbouring expression plasmid
were induced at 42 ℃, and a band of expressed protein of 16 kD was found in
PAGE.
Key Words Hemoglobin； Escherichia coli； gene expression
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