Engineering Novel Functional Proteins： Grafting Active Sites into Natural
Scaffolds
YE Yu-Zhen, TANG Hai-Xu and DING Da-Fu*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract Engineering novel small
functional proteins by grafting active sites into small but stable proteins is
an efficient protein design method. Combining heterogeneous self-consistent
ensemble optimization (hetero-SCEO) with 3D-motif search tool, we developed a
system to accomplish such method. It is tested by transferring zinc-binding
site of carbonic anhydrase form B to charybdotoxin and its efficiency is
demonstrated.
Key Words Functional protein
design；
grafting of active sites； hetero-SCEO； 3D-motif search
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