Doxycycline Dependent Expression of
Epstein-Barr Virus Latent Membrane Protein 1 with Tet Regulating System in
Nasopharygeal Carcinoma Cell Line
LIAO Wei, YI Hong, LI Xiao-Yan, TANG Ming, GU Huan-Hua and
CAO Ya*
( Cancer research Institute of Hunan Medical University, Changsha
410078, China )
Abstract Latent membrane protein
1(LMP1) of Epstein-Barr virus (EBV) plays a vital role in cell transformation
and the nasopharygeal carcinoma (NPC) is highly related to EBV infection. To
elucidate whether LMP1 exerts oncogenic effect in NPC and its possible
mechanism, a dual-stable LMP1 integrated NPC cell line with Tet-on
regulating system were established. Results revealed that expression of LMP-1
could be regulated by Tet system when NPC cell line HNE2 served as
receipt cell, and doxycycline could increase this expression by about 10 fold.
Therefore, it is possible that different level expression of LMP-1
results in its different biological functions and various efforts on
nasopharyngeal carcinogenesis.
Key Words Epstein-Barr virus; latent
membrane protein 1; regulated expression; nasopharyngeal carcinoma
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