New Regulatory Sequence
of Human Connexin 26 Gene
PENG Bai-Lu,ZHANG Xun,ZHOU Ming,ZENG Zhao-Yang and LI
Gui-Yuan*
(Cancer Research Institute,Hunan Medical University,Changsha 410078,China)
Abstract Human connexin 26 gene (Cx26)
has been considered a candidate suppressor gene in mammary epithelial cells.To
explain regulatory mechanism of the Cx26,a DNase-1 hypersensitive 1.6
kb fragment upstream of the 5′-terminus of the gene was sequenced and assayed
with CAT reporter system.The results showed that the 1.6 kb sequence had
powerful promoter activity.The fragment contains two GT boxes (centering at
-6158 and -6213 bp),and a TATA-less TTAAAA box (-6237/-6232 bp) which is the
new promoter region of human Cx26.
Key Words Cx26 gene promoter;sequencing;CAT reporter assay
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