Modification of YACs by Homologous
Recombination and Transfer of a 330 kb YAC into Mammalian Cells
REN Gong-Yi, ZHAO Xin-Tai, WAN Da-Fang and GU Jian-Ren
( National Laboratory of Oncogenes and Related Genes, Shanghai Institute of
Cancer, Shanghai 200032, China )
Abstarct YAC clones were
modified via homologous recombination by transfecting yeast cells containing
YAC with plasmid PRAN4 DNAs . The integration of neo gene into YAC DNA was
identified using Southern blotting and PCR methods. A modified YAC that carried
330 kb human DNA was introduced into L929 cells through PEG mediated
spheroplast fusion, and G418 resistant colonies were obtained.
Key Words YAC transfer； YAC retrofitting； spheroplast fusion
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