LOOH

LOOH, CD, TBARS and REM Kinetic Changes
during Oxidation of High Density Lipoproteins Induced by Cu²⁺ in
Vitro
JIANG Yu and LIU Bing-Wen^*
( Apolipoprotein Research Unit, Institute of Biochemistry and Molecular
Biology,West China University of Medical Sciences, Chengdu 610041, China)

Abstract　　 Oxidation of HDL can be
mediated by Cu²⁺ in vitro. In order to study kinetic
changes of several products during HDL oxidatively modified in vitro,
the kinetic changes of thiobarbituric acid reaction substance (TBARS), lipid
hydroperoxide(LOOH), conjugated diene(CD) and relative electrophoretic
mobility(REM) during the oxidation of HDL induced with Cu²⁺ and HDL
incubation 2-24 hours in vitro were observed. These results indicated
that kinetic changes of LOOH and CD showed three different phases： lag, propagation and
decomposition phases；and the kinetic changes of TBARS and REM
showed two different phases：
lag and propagation phases, but no decomposition phase.
Key Words　　 High density
lipoproteins；
oxidative modification； Cu²⁺； kinetics

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