Relationship between Superoxide Anion Radical and Anion Transport Protein of Red Blood Cell Membrane

Relationship between Superoxide Anion
Radical and Anion Transport Protein of Red Blood Cell Membrane

FU Nai-Yang, ZHANG Er-Xian^*, LIN Zhe-Fu¹
and YU Li-Jun
( Department of Biology, Shantou University, Shantou 515063, China；
¹ Department of Biochemistry, Zhongshan University, Guangzhou
510275, China )

Abstract　　By using a specific inhibitor
of anion transport, DIDS, the dependence on band 3 anion transport protein of
superoxide anion radical (O^–₂^.) flux across red blood cell
(RBC) membrane into RBC was studied； and the effect of
superoxide anion radical on anion transport in RBC membrane was also discussed
based on the hemolytic t_1/2 in an isotonic solution of NH₄Cl.
The results showed that 40 μmol/L DIDS could completely inhibit anion transport
by band 3 protein in RBC membrane, but had no effect on flux into RBC, proving
that the O^–₂^. transport into RBC across RBC
membrane was independent of band 3 protein； the interaction of O^–₂^. with specific SH-group of
membrane protein lowered the anion permeability, and it could be reversed by
DTT and mercaptoethanol.
Key Words　　Superoxide anion radical； band 3 protein； red blood cell

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