High Immunogenicity of the
Pressure-inactivated Virus
TIAN Shao-Min, QIAN Jian-Fei�L, SHAO Guo-Qing�L
and RUAN Kang-Cheng*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract Effects of high hydrostatic
pressure on the activity of infectious bursal disease virus(IBDV) was
described. The infectivity of IBDV decreased with the increasing of pressure
and pressurizing time and may be completely lost, indicated by the tests with
chicken embryo fibrablast cell and young chicken as models. How-ever,
pressure-inactivated IBDV still remained high immunogenicity and might induce high
levels of protecting serum antibody. Fluorescence spectroscopy showed obvious
differences between the structures of native and pressurized IBDV, similar with
the dissociation-reassociation of oligomeric proteins under high pressure. The
changes of pressurized IBDV were also directly observed with electron
microscopy. These results suggest that pressure-inactivated IBDV may be used as
a vaccine.
Key Words High hydrostatic pressure； infectious bursal
disease virus (IBDV)；
immunogenicity； vaccine
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