Molecular Cloning of
Human β-arrestin1 cDNA,
Expression and Functional Study
YU Qing-Ming1,2, CHENG Zhi-Jie1, ZHOU
Tian-Hua1, CEN Bo1, GUO Sheng-Qi2 and PEI Gang1*
( 1Shanghai Institute of Cell Biology, the Chinese Academy of
Sciences, Shanghai 200031, China；2Shanghai Institute of
Biological Products, Ministry of Public Health, Shanghai 200051, China )
Abstract Complete coding sequences of β-arrestin1
(1A and 1B) were cloned through application of bioinformatics analysis to the
dbEST database. β-arrestin1A was overexpressed in E.coli with partial
expression products as inclusion body. Anti-β-arrestin1 antibodies were
prepared by using purified inclusion body. Results also demonstrate that activation of
inhibitory G protein mediated by δ and κ pioid receptors was strongly
attenuated by overexpression of β-arrestin1A in co-transfected 293 cells.
Key Words Bioinformatics； β-arrestin； expression； inclusion body； inhibitory G protein
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