Glucose and Insulin
Regulate Leptin Expression in 3T3-F442A Adipocytes
ANG Fang-Nian, MA Chun-Gu, ZHANG Nai-Xia and SONG Hou-Yan*
( The Laboratory of Molecular Genetics, Shanghai Medical University,
Shanghai 200032, China )
Abstract Relative quantitation RT-PCR
was used to investigate the regulation of leptin expression in 3T3-F442A
adipocytes by glucose and insulin. The results showed that glucose and insulin
stimulated the expression of leptin in 3T3-F442A adipocytes, but they did not
act synergically. Over-high concentration of glucose suppressed the expression
of leptin and the effect of insulin. The elevation of the expression of leptin
was characterized with saturation. The concentration of glucose is very
important for the regulation of leptin expression.
Key Words Obesity; adipocytes; insulin; glucose;
leptin
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