Group II Phospholipase A2
Activity and Its Molecular Regulation
in Rat Heart during Sepsis
JIANG Zhi-Sheng,DONG Lin-Wang,YANG Jun,TONG Li-Jia,PANG
Yong-Zheng,TANG Chao-Shu and LIU Nai-Kui
( Institute of Cardiovascular Basic Research,Beijing Medical
University,Beijing 100083,China )
Abstract Changes of activity and
content of myocardial group II phospholipase A2 and its mRNA
transcription and stability during rat sepsis were investigated. Results showed
that,compared with control group,myocardial group II phospholipase A2
activity in early and late sepsis decreased by 25.0%(P<0.05) and
increased by 47.6%(P<0.01),respectively；group II phospholipase A2
protein concentration reduced by 27.0% and augmented by 48.0%(P<0.01),respectively.
Myocardial group II phospholipase A2 mRNA transcription rate and
content showed similar two-phases changes. The mRNA transcription rate during
early and late sepsis decreased by 45.0% and increased by 70.0%(P<0.01),respectively.
The mRNA content decreased by 34.1% in early sepsis and increased by 157.0% in
late sepsis(P<0.01),respectively. The half-life of group II
phospholipase A2 mRNA remained unchanged notably during early and
late stage of sepsis. These data suggest that myocardial group II phospholipase
A2 activity decreased in early stage of sepsis and increased in its
late stage,and these changes were regulated transcriptionally.
Key Words Group II phospholipase A2；transcription
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