Effect of Protein Kinase C Inhibitor on
Scavenger Receptor
in Human U937 Macrophage-like Cells
JIN Yan,CHEN Qi,WANG Nan,CHEN Xiu-Ying,WEI En-Hui,ZHOU
Chun-Lei and FAN Le-Ming
( Atherosclerosis Research Center,Nanjing Medical University,Nanjing
210029,China )
Abstract In order to investigate
effects of cell protein phosphorylation on scavenger receptor,human U937
macrophage-like cells were treated with protein kinase C inhibitor staurosporine,then
the cells were incubated with ［125I］ox-LDL or ox-LDL ,and the
cellular degradation of ［125I］ox-LDL,its binding to receptor
and the internalization of cell surface ox-LDL receptor complex as well as the
accumulation of lipids within cells were measured separately.Moreover,the
effects of the drug on expression of cell surface receptor were observed by
means of autoradiography.The results indicated that staurosporine could enhance
U937 cells to bind lipids and stimulate scavenger receptor expression,and could
reduce degradation of lipids by U937 cells and the accumulation of cholesterol
within the cells.It suggests that the function of scavenger receptors may be
correlated with cell protein phorsphorylation.
Key Words Scavenger receptor；U937 cell line；protein kinase C inhibitor；ox-LDL
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